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[[Image:1uiv.jpg|left|200px]]


{{Structure
==Crystal structures of the liganded and unliganded nickel binding protein NikA from Escherichia coli (Nickel liganded form)==
|PDB= 1uiv |SIZE=350|CAPTION= <scene name='initialview01'>1uiv</scene>, resolution 1.95&Aring;
<StructureSection load='1uiv' size='340' side='right'caption='[[1uiv]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene>
<table><tr><td colspan='2'>[[1uiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UIV FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
|GENE= NIKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uiv OCA], [https://pdbe.org/1uiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uiv RCSB], [https://www.ebi.ac.uk/pdbsum/1uiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uiv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NIKA_ECOLI NIKA_ECOLI] Involved in a nickel transport system, probably represents the nickel binder.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ui/1uiv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uiv ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacteria have evolved a number of tightly controlled import and export systems to maintain intracellular levels of the essential but potentially toxic metal nickel. Nickel homeostasis systems include the dedicated nickel uptake system nik found in Escherichia coli, a member of the ABC family of transporters, that involves a periplasmic nickel-binding protein, NikA. This is the initial nickel receptor and mediator of the chemotactic response away from nickel. We have solved the crystal structure of NikA protein in the presence and absence of nickel, showing that it behaves as a "classical" periplasmic binding protein. In contrast to other binding proteins, however, the ligand remains accessible to the solvent and is not completely enclosed. No direct bonds are formed between the metal cation and the protein. The nickel binding site is apolar, quite unlike any previously characterized protein nickel binding site. Despite relatively weak binding, NikA is specific for nickel. Using isothermal titration calorimetry, the dissociation constant for nickel was found to be approximately 10 microm and that for cobalt was approximately 20 times higher.


'''Crystal structures of the liganded and unliganded nickel binding protein NikA from Escherichia coli (Nickel liganded form)'''
Crystal structures of the liganded and unliganded nickel-binding protein NikA from Escherichia coli.,Heddle J, Scott DJ, Unzai S, Park SY, Tame JR J Biol Chem. 2003 Dec 12;278(50):50322-9. Epub 2003 Sep 5. PMID:12960164<ref>PMID:12960164</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1uiv" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
Bacteria have evolved a number of tightly controlled import and export systems to maintain intracellular levels of the essential but potentially toxic metal nickel. Nickel homeostasis systems include the dedicated nickel uptake system nik found in Escherichia coli, a member of the ABC family of transporters, that involves a periplasmic nickel-binding protein, NikA. This is the initial nickel receptor and mediator of the chemotactic response away from nickel. We have solved the crystal structure of NikA protein in the presence and absence of nickel, showing that it behaves as a "classical" periplasmic binding protein. In contrast to other binding proteins, however, the ligand remains accessible to the solvent and is not completely enclosed. No direct bonds are formed between the metal cation and the protein. The nickel binding site is apolar, quite unlike any previously characterized protein nickel binding site. Despite relatively weak binding, NikA is specific for nickel. Using isothermal titration calorimetry, the dissociation constant for nickel was found to be approximately 10 microm and that for cobalt was approximately 20 times higher.
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
 
== References ==
==About this Structure==
<references/>
1UIV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UIV OCA].
__TOC__
 
</StructureSection>
==Reference==
Crystal structures of the liganded and unliganded nickel-binding protein NikA from Escherichia coli., Heddle J, Scott DJ, Unzai S, Park SY, Tame JR, J Biol Chem. 2003 Dec 12;278(50):50322-9. Epub 2003 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12960164 12960164]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Heddle, J.]]
[[Category: Heddle J]]
[[Category: Park, S Y.]]
[[Category: Park S-Y]]
[[Category: Scott, D J.]]
[[Category: Scott DJ]]
[[Category: Tame, J R.H.]]
[[Category: Tame JRH]]
[[Category: Unzai, S.]]
[[Category: Unzai S]]
[[Category: NI]]
[[Category: crystal structure]]
[[Category: nickel binding protein]]
[[Category: nickel liganded form]]
 
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