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| ==Crystal structure of potato Rx-CC domain in complex with RanGAP2-WPP domain== | | ==Crystal structure of potato Rx-CC domain in complex with RanGAP2-WPP domain== |
| <StructureSection load='4m70' size='340' side='right' caption='[[4m70]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='4m70' size='340' side='right'caption='[[4m70]], [[Resolution|resolution]] 2.10Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4m70]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Potato Potato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M70 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4m70]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M70 FirstGlance]. <br> |
| </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.103Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato]), RanGAP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato]), RanGAP2, Rx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m70 OCA], [https://pdbe.org/4m70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m70 RCSB], [https://www.ebi.ac.uk/pdbsum/4m70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m70 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m70 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m70 RCSB], [http://www.ebi.ac.uk/pdbsum/4m70 PDBsum]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q9XGF5_SOLTU Q9XGF5_SOLTU] |
| The potato (Solanum tuberosum) disease resistance protein Rx has a modular arrangement that contains coiled-coil (CC), nucleotide-binding (NB), and leucine-rich repeat (LRR) domains and mediates resistance to potato virus X. The Rx N-terminal CC domain undergoes an intramolecular interaction with the Rx NB-LRR region and an intermolecular interaction with the Rx cofactor RanGAP2 (Ran GTPase-activating protein 2). Here, we report the crystal structure of the Rx CC domain in complex with the Trp-Pro-Pro (WPP) domain of RanGAP2. The structure reveals that the Rx CC domain forms a heterodimer with RanGAP2, in striking contrast to the homodimeric structure of the CC domain of the barley disease resistance protein MLA10. Structure-based mutagenesis identified residues from both the Rx CC domain and the RanGAP2 WPP domain that are crucial for their interaction and function in vitro and in vivo. Our results reveal the molecular mechanism underlying the interaction of Rx with RanGAP2 and identify the distinct surfaces of the Rx CC domain that are involved in intramolecular and intermolecular interactions.
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| Structural Basis for the Interaction between the Potato Virus X Resistance Protein (Rx) and Its Cofactor Ran GTPase-activating Protein 2 (RanGAP2).,Hao W, Collier SM, Moffett P, Chai J J Biol Chem. 2013 Dec 13;288(50):35868-76. doi: 10.1074/jbc.M113.517417. Epub, 2013 Nov 5. PMID:24194517<ref>PMID:24194517</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Potato]] | | [[Category: Large Structures]] |
| [[Category: Chai, J]] | | [[Category: Solanum tuberosum]] |
| [[Category: Hao, W]] | | [[Category: Synthetic construct]] |
| [[Category: Coiled coil domain]] | | [[Category: Chai J]] |
| [[Category: Plant disease resistance gene]] | | [[Category: Hao W]] |
| [[Category: Plant protein]]
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| [[Category: Popato x virus]]
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| [[Category: Rangap2]]
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| [[Category: Resistance response]]
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| [[Category: Rx]]
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| [[Category: Wpp domain]]
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