4ilr: Difference between revisions

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==Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6==
==Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6==
<StructureSection load='4ilr' size='340' side='right' caption='[[4ilr]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
<StructureSection load='4ilr' size='340' side='right'caption='[[4ilr]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ilr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ILR FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ilr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ILR FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pkm|3pkm]], [[4ill|4ill]], [[4ilm|4ilm]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.088&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas6b, SSO2004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 Sulfolobus solfataricus P2])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ilr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilr OCA], [https://pdbe.org/4ilr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ilr RCSB], [https://www.ebi.ac.uk/pdbsum/4ilr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ilr ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ilr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ilr RCSB], [http://www.ebi.ac.uk/pdbsum/4ilr PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/CAS6B_SACS2 CAS6B_SACS2] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease, generating an 8 base-long tag known as the 5' handle.<ref>PMID:21507944</ref>  
Clustered regularly interspaced short palindromic repeats (CRISPRs) confer adaptive immunity to prokaryotes through a small RNA-mediated mechanism. Specific endoribonucleases are required by all CRISPR-bearing organisms to process CRISPR RNAs into small RNA that serve as guides for defensive effector complexes. The molecular mechanism of how the endoribonucleases process the class of CRISPR RNA containing no predicted secondary structural features remains largely elusive. Here, we report cocrystal structures of a processing endoribonuclease bound with a noncleavable RNA substrate and its product-like fragment derived from a nonpalindramic repeat. The enzyme stabilizes a short RNA stem-loop structure near the cleavage site and cleaves the phosphodiester bond using an active site comprised of arginine and lysine residues. The distinct RNA binding and cleavage mechanisms underline the diversity in CRISPR RNA processing.
 
Recognition and Cleavage of a Nonstructured CRISPR RNA by Its Processing Endoribonuclease Cas6.,Shao Y, Li H Structure. 2013 Feb 27. pii: S0969-2126(13)00017-8. doi:, 10.1016/j.str.2013.01.010. PMID:23454186<ref>PMID:23454186</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Sulfolobus solfataricus p2]]
[[Category: Large Structures]]
[[Category: Li, H]]
[[Category: Saccharolobus solfataricus P2]]
[[Category: Shao, Y]]
[[Category: Li H]]
[[Category: Hydrolase]]
[[Category: Shao Y]]
[[Category: Rna]]
[[Category: Rna cleavage]]
[[Category: Rrm]]

Latest revision as of 14:57, 1 March 2024

Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6

Structural highlights

4ilr is a 1 chain structure with sequence from Saccharolobus solfataricus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.088Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAS6B_SACS2 CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease, generating an 8 base-long tag known as the 5' handle.[1]

See Also

References

  1. ↑ Lintner NG, Kerou M, Brumfield SK, Graham S, Liu H, Naismith JH, Sdano M, Peng N, She Q, Copie V, Young MJ, White MF, Lawrence CM. Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE). J Biol Chem. 2011 Jun 17;286(24):21643-56. Epub 2011 Apr 20. PMID:21507944 doi:10.1074/jbc.M111.238485

4ilr, resolution 3.09Å

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