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==MYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laser==
==MYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laser==
<StructureSection load='4baj' size='340' side='right' caption='[[4baj]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='4baj' size='340' side='right'caption='[[4baj]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4baj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BAJ FirstGlance]. <br>
<table><tr><td colspan='2'>[[4baj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BAJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ztb|1ztb]], [[4bai|4bai]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4baj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4baj OCA], [https://pdbe.org/4baj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4baj RCSB], [https://www.ebi.ac.uk/pdbsum/4baj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4baj ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chorismate_synthase Chorismate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.5 4.2.3.5] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4baj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4baj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4baj RCSB], [http://www.ebi.ac.uk/pdbsum/4baj PDBsum]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AROC_MYCTU AROC_MYCTU]
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
Line 17: Line 18:
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 4baj" style="background-color:#fffaf0;"></div>
==See Also==
*[[Chorismate synthase|Chorismate synthase]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chorismate synthase]]
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Panjikar, S]]
[[Category: Panjikar S]]
[[Category: Pereira, P J.B]]
[[Category: Pereira PJB]]
[[Category: Royant, A]]
[[Category: Royant A]]
[[Category: Sanctis, D de]]
[[Category: De Sanctis D]]
[[Category: Lyase]]

Latest revision as of 09:22, 17 October 2024

MYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laserMYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laser

Structural highlights

4baj is a 1 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AROC_MYCTU

Publication Abstract from PubMed

Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation- damage induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.

In-house UV radiation damage induced phasing of selenomethionine-labeled protein structures.,Pereira PJ, Royant A, Panjikar S, de Sanctis D J Struct Biol. 2012 Nov 20. pii: S1047-8477(12)00315-2. doi:, 10.1016/j.jsb.2012.11.003. PMID:23178456[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. Pereira PJ, Royant A, Panjikar S, de Sanctis D. In-house UV radiation damage induced phasing of selenomethionine-labeled protein structures. J Struct Biol. 2012 Nov 20. pii: S1047-8477(12)00315-2. doi:, 10.1016/j.jsb.2012.11.003. PMID:23178456 doi:http://dx.doi.org/10.1016/j.jsb.2012.11.003

4baj, resolution 2.30Å

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