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==Crystal structure of B. megaterium levansucrase mutant Y247A==
==Crystal structure of B. megaterium levansucrase mutant Y247A==
<StructureSection load='3om6' size='340' side='right' caption='[[3om6]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
<StructureSection load='3om6' size='340' side='right'caption='[[3om6]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3om6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OM6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OM6 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3om6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OM6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OM6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3om2|3om2]], [[3om4|3om4]], [[3om5|3om5]], [[3om7|3om7]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sacB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3om6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3om6 OCA], [https://pdbe.org/3om6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3om6 RCSB], [https://www.ebi.ac.uk/pdbsum/3om6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3om6 ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3om6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3om6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3om6 RCSB], [http://www.ebi.ac.uk/pdbsum/3om6 PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/D5DC07_PRIM3 D5DC07_PRIM3]
Despite the widespread biological function of carbohydrates, the polysaccharide synthesis mechanisms of glycosyltransferases remain largely unexplored. Bacterial levansucrases (glycoside hydrolase family 68) synthesize high molecular weight, beta-(2,6)-linked levan from sucrose by transfer of fructosyl units. The kinetic and biochemical characterization of Bacillus megaterium levansucrase SacB variants Y247A, Y247W, N252A, D257A, and K373A reveal novel surface motifs remote from the sucrose binding site with distinct influence on the polysaccharide product spectrum. The wild type activity (k(cat)) and substrate affinity (K(m)) are maintained. The structures of the SacB variants reveal clearly distinguishable subsites for polysaccharide synthesis as well as an intact active site architecture. These results lead to a new understanding of polysaccharide synthesis mechanisms. The identified surface motifs are discussed in the context of related glycosyltransferases.
 
Polysaccharide Synthesis of the Levansucrase SacB from Bacillus megaterium Is Controlled by Distinct Surface Motifs.,Strube CP, Homann A, Gamer M, Jahn D, Seibel J, Heinz DW J Biol Chem. 2011 May 20;286(20):17593-600. Epub 2011 Mar 25. PMID:21454585<ref>PMID:21454585</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus megaterium]]
[[Category: Large Structures]]
[[Category: Levansucrase]]
[[Category: Priestia megaterium]]
[[Category: Gamer, M]]
[[Category: Gamer M]]
[[Category: Heinz, D W]]
[[Category: Heinz DW]]
[[Category: Homann, A]]
[[Category: Homann A]]
[[Category: Jahn, D]]
[[Category: Jahn D]]
[[Category: Seibel, J]]
[[Category: Seibel J]]
[[Category: Strube, C P]]
[[Category: Strube CP]]
[[Category: Five fold beta-propeller]]
[[Category: Transferase]]

Latest revision as of 13:35, 21 February 2024

Crystal structure of B. megaterium levansucrase mutant Y247ACrystal structure of B. megaterium levansucrase mutant Y247A

Structural highlights

3om6 is a 4 chain structure with sequence from Priestia megaterium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.96Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

D5DC07_PRIM3

3om6, resolution 1.96Å

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