TolB: Difference between revisions
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<StructureSection load='' size='350' side='right' scene='TolB/Tolb/1' caption='E. coli TolB (PDB code [[1c5k]])'> | |||
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To study the interaction of TolB with Pal, two studies were carried out<ref name='Bouveret'>PMID 7744736</ref>: TolBBep (tagging TolB for immunoprecipitation), which allows the associated proteins to remain in contact with TolB, and ''in vivo'' cross-linking experiments with formaldehyde. Immunoprecipitation gave the result that Pal co-precipitates with TolBBep, while the cross-linking showed that in the present of Pal, the two products migrated close to each other, but in the absence of Pal, neither band was present, demonstrating an interaction between the two. These two experiments showed that TolB directly interacts with Pal, and that this interaction is responsible for maintaining the association of TolB with the membrane. | To study the interaction of TolB with Pal, two studies were carried out<ref name='Bouveret'>PMID 7744736</ref>: TolBBep (tagging TolB for immunoprecipitation), which allows the associated proteins to remain in contact with TolB, and ''in vivo'' cross-linking experiments with formaldehyde. Immunoprecipitation gave the result that Pal co-precipitates with TolBBep, while the cross-linking showed that in the present of Pal, the two products migrated close to each other, but in the absence of Pal, neither band was present, demonstrating an interaction between the two. These two experiments showed that TolB directly interacts with Pal, and that this interaction is responsible for maintaining the association of TolB with the membrane. | ||
{{STRUCTURE_2hqs| PDB=2hqs | SIZE=300| SCENE= |left|CAPTION=E. coli TolB (cyan) complex with peptidoglycan-associated lipoprotein (gold), acetate, sulfate and glycerol, (PDB code = [[2hgs]]) }} | |||
</StructureSection> | </StructureSection> | ||
==3D structures of TolB== | ==3D structures of TolB== | ||
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[[2ivz]] – EcTolB + colicin-E9 T-domain<br /> | [[2ivz]] – EcTolB + colicin-E9 T-domain<br /> | ||
[[4jml]] - EcTolB (mutant) + colicin-E9 T-domain (mutant)<br /> | [[4jml]] - EcTolB (mutant) + colicin-E9 T-domain (mutant)<br /> | ||
[[7nst]] - EcTolB (mutant) + colicin-E9 + outer membrane protein F – Cryo EM<br /> | |||
[[7nsu]] - EcTolB (mutant) + colicin-E9 + outer membrane protein F + BtuB – Cryo EM<br /> | |||
[[3iax]] - EcTolB + colicin-A translocation domain<br /> | [[3iax]] - EcTolB + colicin-A translocation domain<br /> | ||
[[2hqs]], [[2w8b]] - EcTolB + peptidoglycan-associated lipoprotein<br /> | [[2hqs]], [[2w8b]] - EcTolB + peptidoglycan-associated lipoprotein<br /> | ||
[[4pwz]] – | [[4pwz]] – YpTolB – ''Yersinia pestis''<br /> | ||
[[4r40]] - YpTolB + peptidoglycan-associated lipoprotein<br /> | |||
[[6pnv]] – TolB – ''Salmonella enterica''<br /> | |||
[[7mx5]] – TolB – ''Acinetobacteria baumannii''<br /> | |||
== References== | == References== | ||