4x35: Difference between revisions
New page: '''Unreleased structure''' The entry 4x35 is ON HOLD Authors: Roedig, P., Vartiainen, I., Duman, R., Panneerselvam, S., Stuebe, N., Lorbeer, O., Warmer, M., Sutton, G., Stuart, D.I, Wec... |
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==A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering== | |||
<StructureSection load='4x35' size='340' side='right'caption='[[4x35]], [[Resolution|resolution]] 1.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4x35]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Operophtera_brumata_cypovirus_18 Operophtera brumata cypovirus 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X35 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x35 OCA], [https://pdbe.org/4x35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x35 RCSB], [https://www.ebi.ac.uk/pdbsum/4x35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x35 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q30C70_9REOV Q30C70_9REOV] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
At low emittance synchrotron sources it has become possible to perform structure determinations from the measurement of multiple microcrystals which were previously considered too small for diffraction experiments. Conventional mounting techniques do not fulfill the requirements of these new experiments. They significantly contribute to background scattering and it is difficult to locate the crystals, making them incompatible with automated serial crystallography. We have developed a micro-fabricated sample holder from single crystalline silicon with micropores, which carries up to thousands of crystals and significantly reduces the background scattering level. For loading, the suspended microcrystals are pipetted onto the chip and excess mother liquor is subsequently soaked off through the micropores. Crystals larger than the pore size are retained and arrange themselves according to the micropore pattern. Using our chip we were able to collect 1.5 A high resolution diffraction data from protein microcrystals with sizes of 4 micrometers and smaller. | |||
A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering.,Roedig P, Vartiainen I, Duman R, Panneerselvam S, Stube N, Lorbeer O, Warmer M, Sutton G, Stuart DI, Weckert E, David C, Wagner A, Meents A Sci Rep. 2015 May 29;5:10451. doi: 10.1038/srep10451. PMID:26022615<ref>PMID:26022615</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4x35" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Polyhedrin|Polyhedrin]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Operophtera brumata cypovirus 18]] | |||
[[Category: David C]] | |||
[[Category: Duman R]] | |||
[[Category: Lorbeer O]] | |||
[[Category: Meents A]] | |||
[[Category: Panneerselvam S]] | |||
[[Category: Roedig P]] | |||
[[Category: Stuart DI]] | |||
[[Category: Stuebe N]] | |||
[[Category: Sutton G]] | |||
[[Category: Vartiainen I]] | |||
[[Category: Wagner A]] | |||
[[Category: Warmer M]] | |||
[[Category: Weckert E]] | |||