3thk: Difference between revisions

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 ==Structure of SH3 chimera with a type II ligand linked to the chain C-terminal==
-<StructureSection load='3thk' size='340' side='right' caption='[[3thk]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+<StructureSection load='3thk' size='340' side='right'caption='[[3thk]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3thk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2pqh 2pqh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3THK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3THK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3thk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2pqh 2pqh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3THK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3THK FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pwt|1pwt]], [[1shg|1shg]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sptan1, Spna2, Spta2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3thk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3thk OCA], [https://pdbe.org/3thk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3thk RCSB], [https://www.ebi.ac.uk/pdbsum/3thk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3thk ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3thk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3thk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3thk RCSB], [http://www.ebi.ac.uk/pdbsum/3thk PDBsum]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/SPTN1_RAT SPTN1_RAT] Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
-A new chimeric protein, named WT-CIIA, was designed by connecting the proline-rich decapeptide PPPVPPYSAG to the C-terminus of the alpha-spectrin SH3 domain through a natural twelve-residue linker to obtain a single-chain model that would imitate intramolecular SH3-ligand interaction. The crystal structure of this fusion protein was determined at 1.7 A resolution. The asymmetric unit of the crystal contained two SH3 globules contacting with one PPPVPPY fragment located between them. The domains are related by the two-fold non-crystallographic axis and the ligand lies in two opposite orientations with respect to the conservative binding sites of SH3 domains.
-High-Resolution Crystal Structure of Spectrin SH3 Domain Fused with a Proline-Rich Peptide.,Gushchina LV, Gabdulkhakov AG, Nikonov SV, Filimonov VV J Biomol Struct Dyn. 2011 Dec;29(3):485-95. PMID:22066535<ref>PMID:22066535</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
 ==See Also==
-*[[Spectrin|Spectrin]]
+*[[Spectrin 3D structures|Spectrin 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Rattus norvegicus]]
-[[Category: Filimonov, V V]]
+[[Category: Filimonov VV]]
-[[Category: Gabdulkhakov, A G]]
+[[Category: Gabdulkhakov AG]]
-[[Category: Gushchina, L V]]
+[[Category: Gushchina LV]]
-[[Category: Nikonov, S V]]
+[[Category: Nikonov SV]]
-[[Category: Nikulin, A D]]
+[[Category: Nikulin AD]]
-[[Category: Chimera]]
-[[Category: Sh3 domain]]
-[[Category: Structural protein]]