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==Crystal structure of the tyrosine kinase binding domain of Cbl-c (PL mutant)==
==Crystal structure of the tyrosine kinase binding domain of Cbl-c (PL mutant)==
<StructureSection load='3vrq' size='340' side='right' caption='[[3vrq]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
<StructureSection load='3vrq' size='340' side='right'caption='[[3vrq]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3vrq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VRQ FirstGlance]. <br>
<table><tr><td colspan='2'>[[3vrq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VRQ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.39&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vrn|3vrn]], [[3vro|3vro]], [[3vrp|3vrp]], [[3vrr|3vrr]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CBLC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vrq OCA], [https://pdbe.org/3vrq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vrq RCSB], [https://www.ebi.ac.uk/pdbsum/3vrq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vrq ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vrq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vrq RCSB], [http://www.ebi.ac.uk/pdbsum/3vrq PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/CBLC_HUMAN CBLC_HUMAN] Regulator of EGFR mediated signal transduction.
Through their ubiquitin ligase activity, Cbl-family proteins suppress signalling mediated by protein-tyrosine kinases (PTKs), but can also function as adaptor proteins to positively regulate signalling. The tyrosine kinase binding (TKB) domain of this family is critical for binding with tyrosine-phosphorylated target proteins. Here, we analysed the crystal structure of the TKB domain of Cbl-c/Cbl-3 (Cbl-c TKB), which is a distinct member of the mammalian Cbl-family. In comparison with Cbl TKB, Cbl-c TKB showed restricted structural flexibility upon phosphopeptide binding. A mutation in Cbl-c TKB augmenting this flexibility enhanced its binding to target phosphoproteins. These results suggest that proteins, post-translational modifications or mutations that alter structural flexibility of the TKB domain of Cbl-family proteins could regulate their binding to target phosphoproteins and thereby, affect PTK-mediated signalling.
 
Structural flexibility regulates phosphopeptide-binding activity of the tyrosine kinase binding domain of Cbl-c.,Takeshita K, Tezuka T, Isozaki Y, Yamashita E, Suzuki M, Kim M, Yamanashi Y, Yamamoto T, Nakagawa A J Biochem. 2012 Nov;152(5):487-95. doi: 10.1093/jb/mvs085. Epub 2012 Aug 9. PMID:22888118<ref>PMID:22888118</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Isozaki, Y]]
[[Category: Large Structures]]
[[Category: Nakagawa, A]]
[[Category: Isozaki Y]]
[[Category: Suzuki, M]]
[[Category: Nakagawa A]]
[[Category: Takeshita, K]]
[[Category: Suzuki M]]
[[Category: Tezuka, T]]
[[Category: Takeshita K]]
[[Category: Yamamoto, T]]
[[Category: Tezuka T]]
[[Category: Yamanashi, Y]]
[[Category: Yamamoto T]]
[[Category: Yamashita, E]]
[[Category: Yamanashi Y]]
[[Category: Calcium-binding ef hand]]
[[Category: Yamashita E]]
[[Category: Divergent sh2 domain]]
[[Category: Protein binding]]
[[Category: Ptb domain]]
[[Category: Regulator of egfr mediated signal transduction]]
[[Category: Ubiquitously expressed]]

Latest revision as of 11:41, 20 March 2024

Crystal structure of the tyrosine kinase binding domain of Cbl-c (PL mutant)Crystal structure of the tyrosine kinase binding domain of Cbl-c (PL mutant)

Structural highlights

3vrq is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.39Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CBLC_HUMAN Regulator of EGFR mediated signal transduction.

3vrq, resolution 2.39Å

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