1m7u: Difference between revisions

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[[Image:1m7u.jpg|left|200px]]


{{Structure
==Crystal structure of a novel DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast==
|PDB= 1m7u |SIZE=350|CAPTION= <scene name='initialview01'>1m7u</scene>, resolution 2.8&Aring;
<StructureSection load='1m7u' size='340' side='right'caption='[[1m7u]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1m7u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M7U FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
|GENE= Ndt80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m7u OCA], [https://pdbe.org/1m7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m7u RCSB], [https://www.ebi.ac.uk/pdbsum/1m7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m7u ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Crystal structure of a novel DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast'''
[https://www.uniprot.org/uniprot/NDT80_YEAST NDT80_YEAST] Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation.<ref>PMID:8524222</ref> <ref>PMID:9660952</ref> <ref>PMID:9742092</ref> <ref>PMID:12832469</ref>
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
Ndt80 is a transcriptional activator required for meiosis in the yeast Saccharomyces cerevisiae. Here, we report the crystal structure at 2.3 A resolution of the DNA-binding domain of Ndt80 experimentally phased by using the anomalous and isomorphous signal from a single ordered Se atom per molecule of 272-aa residues. The structure reveals a single approximately 32-kDa domain with a distinct fold comprising a beta-sandwich core elaborated with seven additional beta-sheets and three short alpha-helices. Inspired by the structure, we have performed a mutational analysis and defined a DNA-binding motif in this domain. The DNA-binding domain of Ndt80 is homologous to a number of proteins from higher eukaryotes, and the residues that we have shown are required for DNA binding by Ndt80 are highly conserved among this group of proteins. These results suggest that Ndt80 is the defining member of a previously uncharacterized family of transcription factors, including the human protein (C11orf9), which has been shown to be highly expressed in invasive or metastatic tumor cells.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m7/1m7u_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1M7U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7U OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m7u ConSurf].
Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast., Montano SP, Cote ML, Fingerman I, Pierce M, Vershon AK, Georgiadis MM, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14041-6. Epub 2002 Oct 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12384578 12384578]
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Cote ML]]
[[Category: Cote, M L.]]
[[Category: Fingerman I]]
[[Category: Fingerman, I.]]
[[Category: Georgiadis MM]]
[[Category: Georgiadis, M M.]]
[[Category: Montano SP]]
[[Category: Montano, S P.]]
[[Category: Pierce M]]
[[Category: Pierce, M.]]
[[Category: Vershon AK]]
[[Category: Vershon, A K.]]
[[Category: dna-binding]]
[[Category: meiosis]]
[[Category: transcription activator]]
[[Category: yeast protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:40:03 2008''

Latest revision as of 10:41, 14 February 2024

Crystal structure of a novel DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeastCrystal structure of a novel DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast

Structural highlights

1m7u is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NDT80_YEAST Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation.[1] [2] [3] [4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Xu L, Ajimura M, Padmore R, Klein C, Kleckner N. NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae. Mol Cell Biol. 1995 Dec;15(12):6572-81. PMID:8524222
  2. ↑ Chu S, Herskowitz I. Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80. Mol Cell. 1998 Apr;1(5):685-96. PMID:9660952
  3. ↑ Hepworth SR, Friesen H, Segall J. NDT80 and the meiotic recombination checkpoint regulate expression of middle sporulation-specific genes in Saccharomyces cerevisiae. Mol Cell Biol. 1998 Oct;18(10):5750-61. PMID:9742092
  4. ↑ Pierce M, Benjamin KR, Montano SP, Georgiadis MM, Winter E, Vershon AK. Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression. Mol Cell Biol. 2003 Jul;23(14):4814-25. PMID:12832469

1m7u, resolution 2.80Å

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