Nisin: Difference between revisions

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Tal stern, Michal Harel, Jaime Prilusky

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-[[Image:<math>Example.jpg]][[<scene name='60/607847/Nisin_lipid_complex/1'>TextToBeDisplayed</scene>]]==Nisin-lipid || complex==
+== Nisin ==
-<StructureSection <math>[[Media:Insert formula here]]</math>load='1stp' size='340' side='right' caption='60/607847/Nisin_structure/4' scene=''>
-This is a default text for your page '''Tal stern/Test page'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
-You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.
-[[nisin]] is a polycylic antobacterial peptid, with 34 amino acid residues used as a food preservative.
+<StructureSection load='1wco' size='340' side='right' caption='60/607847/Nisin_structure/4 in complex with lipid (PDB code [[1wco]])' scene=''>
-Nisin is produced by fermentation using the bacterium Lactococcus lactis. In the food industry, it is obtained from the culturing of L. lactis on natural substrates, such as milk or dextrose, and is not chemically synthesized.
+== Function ==
-While in general most [[bacteriocins]] inhibit only closely related species, nisin is a rare example of a "broad-spectrum" bacteriocin effective against many Gram-positive organisms, including lactic acid bacteria (commonly associated with spoilage), Listeria monocytogenes (a known pathogen), Staphylococcus aureus, Bacillus cereus, Clostridium botulinum, etc. It is also particularly effective against spores. Gram-negative bacteria are protected by their outer membrane but may become susceptible to nisin action after a heat shock or when this is coupled with the chelator EDTA. Nisin is soluble in water and can be effective at levels nearing the parts-per-billion range.
-== Function ==
+'''Nisin''' is a polycylic antibacterial peptide, with 34 amino acid residues used as a food preservative.
+Nisin is produced by fermentation using the bacterium ''Lactococcus lactis''.
-== Disease ==
+While in general most bacteriocins inhibit only closely related species, nisin is a rare example of a "broad-spectrum" bacteriocin effective against many Gram-positive organisms, including lactic acid bacteria (commonly associated with spoilage), ''Listeria monocytogenes'' (a known pathogen), ''Staphylococcus aureus,'' ''Bacillus cereus'', ''Clostridium botulinum'', etc. It is also particularly effective against spores. Gram-negative bacteria are protected by their outer membrane but may become susceptible to nisin action after a heat shock or when this is coupled with the chelator EDTA. Nisin is soluble in water and can be effective at levels nearing the parts-per-billion range<ref>PMID:2119570</ref>.
 == Relevance ==
+In the food industry, it is obtained from the culturing of ''L. lactis'' on natural substrates, such as milk or dextrose, and is not chemically synthesized.  Nisin is studied as a novel antibiotic due to its antimicrobial activity and as a therapeutic against neck and head squamous cell carcinoma<ref>PMID:26132406</ref>.
 == Structural highlights ==
 This is a sample scene created with SAT to <scene name='60/607847/Nisin_lipid_complex/7'>color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
-<scene name='60/607847/Nisin_lipid_complex/8'>Text Displayed On Page</scene>
+the structure of nisin that is available here is complex of nisin with lipid II. The origin of this structure is from lactococcus lactis.
+<scene name='60/607847/Nisin_lipid_complex/8'>nisin lipid II complex</scene>
+<scene name='60/607847/Nisin_backbone/1'>NIsin backbone</scene>
+Basic structure of nisin is presented <scene name='60/607847/Basic_structure/1'>here</scene>. You can see the chain fron N (blue) to C (red).
+<scene name='60/607847/Spacefill/1'>Here</scene> you can see the peptid in a spacefill presentation.
 </StructureSection>
+== 3D structure of nisin ==
+Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+[[5xhb]] - LlNisin - ''Lactococcus lactis'' <br />
+[[1wco]] - LlNisin + lipid - NMR<br />
+[[2n32]] - LlNisin N-terminal - NMR<br />
+[[2n2e]] - LlNisin C-terminal - NMR<br />
 == References ==
 <references/>
+[[Category:Topic Page]]