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==Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae== | |||
<StructureSection load='1lmv' size='340' side='right'caption='[[1lmv]]' scene=''> | |||
== Structural highlights == | |||
| | <table><tr><td colspan='2'>[[1lmv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LMV FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lmv OCA], [https://pdbe.org/1lmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lmv RCSB], [https://www.ebi.ac.uk/pdbsum/1lmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lmv ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
''' | == Publication Abstract from PubMed == | ||
== | |||
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing. | Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing. | ||
Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.,Newby MI, Greenbaum NL Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583<ref>PMID:12426583</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1lmv" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Greenbaum | [[Category: Greenbaum NL]] | ||
[[Category: Newby | [[Category: Newby MI]] | ||
Latest revision as of 21:48, 29 November 2023
Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiaeSolution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae
Structural highlights
Publication Abstract from PubMedPairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing. Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.,Newby MI, Greenbaum NL Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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