CCA-adding enzyme: Difference between revisions

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<StructureSection load='3ovs' size='350' side='right' caption='Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry [[3ovs]])' scene=''>
<StructureSection load='3ovs' size='350' side='right' caption='Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry [[3ovs]])' scene='55/551188/Cv/1'>
      
      
'''CCA-adding enzyme''' (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA.  CAE has one active site which is capable of binding CTP and ATP in a specific sequence.  In the bacteria Aquifex aeolicus, CAE consists of 2 subunits.  One specific for CTP and the other for ATP.  CAE builds the CCA without a genetic template.
'''CCA-adding enzyme''' (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA.  CAE has one active site which is capable of binding CTP and ATP in a specific sequence.  In the bacteria Aquifex aeolicus, CAE consists of 2 subunits.  One specific for CTP and the other for ATP.  CAE builds the CCA without a genetic template.<ref>PMID:15295590</ref>
</StructureSection>
*<scene name='55/551188/Cv/4'>CCA-adding enzyme complex with tRNA, CTP, ethanediol and Ca+2 ion</scene> (PDB entry [[3ovs]]).
*<scene name='55/551188/Cv/5'>Ca+2 coordination site of CCA-adding enzyme</scene> (PDB entry [[3ovs]]).<ref>PMID:21071662</ref>


==3D structures of CCA-adding enzyme==
==3D structures of CCA-adding enzyme==
[[CCA-adding enzyme 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*CCA-adding enzyme
 
**[[1miv]] – BsCAE – ''Bacillus stearothermophilus''<BR />
**[[1uet]], [[1r8a]] – AfCAE – ''Archaeoglobus fulgidus''<BR />
**[[1ou5]] – hCAE – human<br />
 
*CCA-adding enzyme binary complex with nucleotide
 
**[[1miw]] – BsCAE + ATP<BR />
**[[1uev]], [[1r8b]] – AfCAE + ATP<BR />
**[[1miy]] – BsCAE + CTP<BR />
**[[1ueu]], [[1r89]] – AfCAE + CTP<BR />
**[[1r8c]] – AfCAE + UTP<BR />
 
*CCA-adding enzyme binary complex with tRNA
 
**[[2dr5]] – AfCAE + tRNA 32-mer<BR />
**[[2dr7]], [[2zh1]], [[2zh7]], [[2zh9]] – AfCAE + tRNA 33-mer<BR />
**[[2dr9]], [[2zh2]], [[2zh3]], [[2zh4]], [[2zh5]], [[2zh8]], [[2zhb]] – AfCAE + tRNA 34-mer<BR />
**[[2drb]], [[3ouy]] – AfCAE + tRNA 35-mer<BR />


*CCA-adding enzyme ternary complex with nucleotide and tRNA


**[[1sz1]] – AfCAE + GTP + UMP + CMP + tRNA<BR />
== References ==
**[[1tfw]] – AfCAE + ATP + tRNA 3’ end<BR />
<references/>
**[[1tfy]] – AfCAE + CTP + tRNA 3’ end<BR />
**[[2dr8]], [[2zha]] – AfCAE + CTP + tRNA 33-mer<BR />
**[[2dra]], [[2zh6]], [[3ov7]] – AfCAE + ATP + tRNA 34-mer<BR />
**[[3ova]] – AfCAE + ATP derivative + tRNA 34-mer<BR />
**[[2dvi]], [[3ovs]] – AfCAE + CTP + tRNA 34-mer<BR />
**[[3ovb]] – AfCAE + ATP + tRNA 35-mer<BR />
}}
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 11:54, 30 April 2019


CCA-adding enzyme (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA. CAE has one active site which is capable of binding CTP and ATP in a specific sequence. In the bacteria Aquifex aeolicus, CAE consists of 2 subunits. One specific for CTP and the other for ATP. CAE builds the CCA without a genetic template.[1]

3D structures of CCA-adding enzyme

CCA-adding enzyme 3D structures


Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry 3ovs)

Drag the structure with the mouse to rotate


ReferencesReferences

  1. ↑ Xiong Y, Steitz TA. Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template. Nature. 2004 Aug 5;430(7000):640-5. PMID:15295590 doi:http://dx.doi.org/10.1038/nature02711
  2. ↑ Pan B, Xiong Y, Steitz TA. How the CCA-adding enzyme selects adenine over cytosine at position 76 of tRNA. Science. 2010 Nov 12;330(6006):937-40. PMID:21071662 doi:10.1126/science.1194985

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Michal Harel, Alexander Berchansky
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