1k8k: Difference between revisions

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-[[Image:1k8k.gif|left|200px]]
- {{Structure
+==Crystal Structure of Arp2/3 Complex==
-|PDB= 1k8k |SIZE=350|CAPTION= <scene name='initialview01'>1k8k</scene>, resolution 2.0&Aring;
+<StructureSection load='1k8k' size='340' side='right'caption='[[1k8k]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[1k8k]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K8K FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-|GENE=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k8k OCA], [https://pdbe.org/1k8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k8k RCSB], [https://www.ebi.ac.uk/pdbsum/1k8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k8k ProSAT]</span></td></tr>
-}}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ARP3_BOVIN ARP3_BOVIN] Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/1k8k_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k8k ConSurf].
+<div style="clear:both"></div>
-'''Crystal Structure of Arp2/3 Complex'''
+==See Also==
+*[[Actin-related protein 3D structures|Actin-related protein 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-We determined a crystal structure of bovine Arp2/3 complex, an assembly of seven proteins that initiates actin polymerization in eukaryotic cells, at 2.0 angstrom resolution. Actin-related protein 2 (Arp2) and Arp3 are folded like actin, with distinctive surface features. Subunits ARPC2 p34 and ARPC4 p20 in the core of the complex associate through long carboxyl-terminal alpha helices and have similarly folded amino-terminal alpha/beta domains. ARPC1 p40 is a seven-blade beta propeller with an insertion that may associate with the side of an actin filament. ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament.
-==About this Structure==
-K8K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8K OCA].
-==Reference==
-Crystal structure of Arp2/3 complex., Robinson RC, Turbedsky K, Kaiser DA, Marchand JB, Higgs HN, Choe S, Pollard TD, Science. 2001 Nov 23;294(5547):1679-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11721045 11721045]
 [[Category: Bos taurus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Choe, S.]]
+[[Category: Choe S]]
-[[Category: Higgs, H N.]]
+[[Category: Higgs HN]]
-[[Category: Kaiser, D A.]]
+[[Category: Kaiser DA]]
-[[Category: Marchand, J B.]]
+[[Category: Marchand J-B]]
-[[Category: Pollard, T D.]]
+[[Category: Pollard TD]]
-[[Category: Robinson, R C.]]
+[[Category: Robinson RC]]
-[[Category: Turbedsky, K.]]
+[[Category: Turbedsky K]]
-[[Category: beta-propeller]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:13:57 2008''