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[[Image:1jqq.jpg|left|200px]]


{{Structure
==Crystal structure of Pex13p(301-386) SH3 domain==
|PDB= 1jqq |SIZE=350|CAPTION= <scene name='initialview01'>1jqq</scene>, resolution 2.65&Aring;
<StructureSection load='1jqq' size='340' side='right'caption='[[1jqq]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND=
<table><tr><td colspan='2'>[[1jqq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JQQ FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqq OCA], [https://pdbe.org/1jqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jqq RCSB], [https://www.ebi.ac.uk/pdbsum/1jqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jqq ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Crystal structure of Pex13p(301-386) SH3 domain'''
[https://www.uniprot.org/uniprot/PEX13_YEAST PEX13_YEAST] Component of the peroxisomal translocation machinery with PEX14 and PEX17. Interacts with the PTS1 receptor (PAS10/PEX5).
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combined use of X-ray crystallography, NMR spectroscopy, and circular dichroism, we show that the two ligands bind in unrelated conformations to patches located at opposite surfaces of this SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions within the Pex13p-Pex5p interface specifically impair PTS1-dependent protein import into yeast peroxisomes.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/1jqq_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1JQQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQQ OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jqq ConSurf].
Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain., Douangamath A, Filipp FV, Klein AT, Barnett P, Zou P, Voorn-Brouwer T, Vega MC, Mayans OM, Sattler M, Distel B, Wilmanns M, Mol Cell. 2002 Nov;10(5):1007-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12453410 12453410]
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Barnett P]]
[[Category: Barnett, P.]]
[[Category: Distel B]]
[[Category: Distel, B.]]
[[Category: Douangamath A]]
[[Category: Douangamath, A.]]
[[Category: Mayans O]]
[[Category: Mayans, O.]]
[[Category: Wilmanns M]]
[[Category: Wilmanns, M.]]
[[Category: compact beta-barrel of five anti-parrallel beta-strand]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:07 2008''

Latest revision as of 10:41, 7 February 2024

Crystal structure of Pex13p(301-386) SH3 domainCrystal structure of Pex13p(301-386) SH3 domain

Structural highlights

1jqq is a 4 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.65Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PEX13_YEAST Component of the peroxisomal translocation machinery with PEX14 and PEX17. Interacts with the PTS1 receptor (PAS10/PEX5).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1jqq, resolution 2.65Å

Drag the structure with the mouse to rotate

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OCA
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