1i52: Difference between revisions

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-[[Image:1i52.gif|left|200px]]
- {{Structure
+==CRYSTAL STRUCTURE OF 4-DIPHOSPHOCYTIDYL-2-C-METHYLERYTHRITOL (CDP-ME) SYNTHASE (YGBP) INVOLVED IN MEVALONATE INDEPENDENT ISOPRENOID BIOSYNTHESIS==
-|PDB= 1i52 |SIZE=350|CAPTION= <scene name='initialview01'>1i52</scene>, resolution 1.50&Aring;
+<StructureSection load='1i52' size='340' side='right'caption='[[1i52]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=CTP:CYTIDINE-5'-TRIPHOSPHATE'>CTP</scene>
+<table><tr><td colspan='2'>[[1i52]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I52 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-|GENE= ISPD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i52 OCA], [https://pdbe.org/1i52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i52 RCSB], [https://www.ebi.ac.uk/pdbsum/1i52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i52 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ISPD_ECOLI ISPD_ECOLI] Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).[HAMAP-Rule:MF_00108]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/1i52_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i52 ConSurf].
+<div style="clear:both"></div>
-'''CRYSTAL STRUCTURE OF 4-DIPHOSPHOCYTIDYL-2-C-METHYLERYTHRITOL (CDP-ME) SYNTHASE (YGBP) INVOLVED IN MEVALONATE INDEPENDENT ISOPRENOID BIOSYNTHESIS'''
+==See Also==
+*[[MEP cytidylyltransferase|MEP cytidylyltransferase]]
+*[[MEP cytidylyltransferase 3D structures|MEP cytidylyltransferase 3D structures]]
-==Overview==
+__TOC__
-The YgbP protein of Escherichia coli encodes the enzyme 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) synthetase, a member of the cytidyltransferase family of enzymes. CDP-ME is an intermediate in the mevalonate-independent pathway for isoprenoid biosynthesis in a number of prokaryotic organisms, algae, the plant plastids and the malaria parasite. Because vertebrates synthesize isoprenoid precursors using a mevalonate pathway, CDP-ME synthetase and other enzymes of the mevalonate-independent pathway for isoprenoid production represent attractive targets for the structure-based design of selective antibacterial, herbicidal and antimalarial drugs. The high-resolution structures of E. coli CDP-ME synthetase in the apo form and complexed with both CTP-Mg2+ and CDP-ME-Mg2+ reveal the stereochemical principles underlying both substrate and product recognition as well as catalysis in CDP-ME synthetase. Moreover, these complexes represent the first experimental structures for any cytidyltransferase with both substrates and products bound.
+</StructureSection>
+[[Category: Escherichia coli K-12]]
-==About this Structure==
+[[Category: Large Structures]]
-I52 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I52 OCA].
+[[Category: Bowman ME]]
+[[Category: Cane DE]]
-==Reference==
+[[Category: Chow C]]
-Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis., Richard SB, Bowman ME, Kwiatkowski W, Kang I, Chow C, Lillo AM, Cane DE, Noel JP, Nat Struct Biol. 2001 Jul;8(7):641-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11427897 11427897]
+[[Category: Kang I]]
-[[Category: Escherichia coli]]
+[[Category: Kwiatkowski W]]
-[[Category: Single protein]]
+[[Category: Lillo M]]
-[[Category: Bowman, M E.]]
+[[Category: Noel JP]]
-[[Category: Cane, D E.]]
+[[Category: Richard SB]]
-[[Category: Chow, C.]]
-[[Category: Kang, I.]]
-[[Category: Kwiatkowski, W.]]
-[[Category: Lillo, M.]]
-[[Category: Noel, J P.]]
-[[Category: Richard, S B.]]
-[[Category: CA]]
-[[Category: CTP]]
-[[Category: MG]]
-[[Category: cytidylyltransferase]]
-[[Category: deoxyxylulose-5-phosphate pathway (dxp)]]
-[[Category: isoprenoid biosynthesy]]
-[[Category: mep]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:45:38 2008''