7ahl: Difference between revisions

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New page: ==ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS== <StructureSection load='7ahl' size='340' side='right' caption='7ahl, resolution 1.89Å' scene=''> == Structural highl...
 
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==ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS==
==ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS==
<StructureSection load='7ahl' size='340' side='right' caption='[[7ahl]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
<StructureSection load='7ahl' size='340' side='right'caption='[[7ahl]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7AHL FirstGlance]. <br>
<table><tr><td colspan='2'>[[7ahl]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AHL FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [http://www.ebi.ac.uk/pdbsum/7ahl PDBsum]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89&#8491;</td></tr>
<table>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ahl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ahl OCA], [https://pdbe.org/7ahl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ahl RCSB], [https://www.ebi.ac.uk/pdbsum/7ahl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ahl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HLA_STAAU HLA_STAAU] Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/7ahl_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/7ahl_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7ahl ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the Staphylococcus aureus alpha-hemolysin pore has been determined to 1.9 A resolution. Contained within the mushroom-shaped homo-oligomeric heptamer is a solvent-filled channel, 100 A in length, that runs along the sevenfold axis and ranges from 14 A to 46 A in diameter. The lytic, transmembrane domain comprises the lower half of a 14-strand antiparallel beta barrel, to which each protomer contributes two beta strands, each 65 A long. The interior of the beta barrel is primarily hydrophilic, and the exterior has a hydrophobic belt 28 A wide. The structure proves the heptameric subunit stoichiometry of the alpha-hemolysin oligomer, shows that a glycine-rich and solvent-exposed region of a water-soluble protein can self-assemble to form a transmembrane pore of defined structure, and provides insight into the principles of membrane interaction and transport activity of beta barrel pore-forming toxins.
Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore.,Song L, Hobaugh MR, Shustak C, Cheley S, Bayley H, Gouaux JE Science. 1996 Dec 13;274(5294):1859-66. PMID:8943190<ref>PMID:8943190</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Hemolysin|Hemolysin]]
*[[Hemolysin|Hemolysin]]
*[[Hemolysin 3D structures|Hemolysin 3D structures]]
*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
*[[User:Eric Martz/JSmol Notes|User:Eric Martz/JSmol Notes]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Bayley, H.]]
[[Category: Bayley H]]
[[Category: Cheley, S.]]
[[Category: Cheley S]]
[[Category: Gouaux, J E.]]
[[Category: Gouaux JE]]
[[Category: Hobaugh, M.]]
[[Category: Hobaugh M]]
[[Category: Shustak, C.]]
[[Category: Shustak C]]
[[Category: Song, L.]]
[[Category: Song L]]
[[Category: Cytolytic protein]]
[[Category: Hemolysin]]
[[Category: Transmembrane pore]]

Latest revision as of 17:51, 6 March 2024

ALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUSALPHA-HEMOLYSIN FROM STAPHYLOCOCCUS AUREUS

Structural highlights

7ahl is a 7 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.89Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HLA_STAAU Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

7ahl, resolution 1.89Å

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