1s49: Difference between revisions
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==Crystal Structure of RNA-dependent RNA polymerase construct 1 (residues 71-679) from bovine viral diarrhea virus complexed with GTP== | ==Crystal Structure of RNA-dependent RNA polymerase construct 1 (residues 71-679) from bovine viral diarrhea virus complexed with GTP== | ||
<StructureSection load='1s49' size='340' side='right' caption='[[1s49]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='1s49' size='340' side='right'caption='[[1s49]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1s49]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1s49]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_viral_diarrhea_virus_1 Bovine viral diarrhea virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S49 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | ||
<tr><td class="sblockLbl"><b>[[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s49 OCA], [https://pdbe.org/1s49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s49 RCSB], [https://www.ebi.ac.uk/pdbsum/1s49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s49 ProSAT]</span></td></tr> | |||
<tr | </table> | ||
== Function == | |||
<table> | [https://www.uniprot.org/uniprot/POLG_BVDVN POLG_BVDVN] Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans. E1 and/or E2 are responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis (Probable).<ref>PMID:14963137</ref> <ref>PMID:16051874</ref> P7 forms a leader sequence to properly orient NS2 in the membrane (By similarity).<ref>PMID:14963137</ref> <ref>PMID:16051874</ref> Uncleaved NS2-3 is required for production of infectious virus.<ref>PMID:14963137</ref> <ref>PMID:16051874</ref> NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.<ref>PMID:14963137</ref> <ref>PMID:16051874</ref> NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase.<ref>PMID:14963137</ref> <ref>PMID:16051874</ref> NS4A is a cofactor for the NS3 protease activity (By similarity).<ref>PMID:14963137</ref> <ref>PMID:16051874</ref> RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.<ref>PMID:14963137</ref> <ref>PMID:16051874</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s4/1s49_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s4/1s49_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s49 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 1s49" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[RNA polymerase|RNA polymerase]] | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Bovine viral diarrhea virus 1]] | [[Category: Bovine viral diarrhea virus 1]] | ||
[[Category: Choi | [[Category: Large Structures]] | ||
[[Category: Groarke | [[Category: Choi KH]] | ||
[[Category: Kuhn | [[Category: Groarke JM]] | ||
[[Category: Pevear | [[Category: Kuhn RJ]] | ||
[[Category: Rossmann | [[Category: Pevear DC]] | ||
[[Category: Smith | [[Category: Rossmann MG]] | ||
[[Category: Young | [[Category: Smith JL]] | ||
[[Category: Young DC]] | |||