1en7: Difference between revisions

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-[[Image:1en7.jpg|left|200px]]
- {{Structure
+==ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4==
-|PDB= 1en7 |SIZE=350|CAPTION= <scene name='initialview01'>1en7</scene>, resolution 2.4&Aring;
+<StructureSection load='1en7' size='340' side='right'caption='[[1en7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
+<table><tr><td colspan='2'>[[1en7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EN7 FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-|GENE= GP49 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T4])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1en7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1en7 OCA], [https://pdbe.org/1en7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1en7 RCSB], [https://www.ebi.ac.uk/pdbsum/1en7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1en7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/END7_BPT4 END7_BPT4] Cleaves DNA cruciform and Y-structures as well as heteroduplex loops. Resolves Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/en/1en7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1en7 ConSurf].
+<div style="clear:both"></div>
-'''ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4'''
+==See Also==
+*[[Endonuclease 3D structures|Endonuclease 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-Phage T4 endonuclease VII (Endo VII), the first enzyme shown to resolve Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches. We have determined the crystal structures of the Ca2+-bound wild-type and the inactive N62D mutant enzymes at 2.4 and 2.1 A, respectively. The Endo VII monomers form an elongated, highly intertwined molecular dimer exhibiting extreme domain swapping. The major dimerization elements are two pairs of antiparallel helices forming a novel 'four-helix cross' motif. The unique monomer fold, almost completely lacking beta-sheet structure and containing a zinc ion tetrahedrally coordinated to four cysteines, does not resemble any of the known junction-resolving enzymes, including the Escherichia coli RuvC and lambda integrase-type recombinases. The S-shaped dimer has two 'binding bays' separated by approximately 25 A which are lined by positively charged residues and contain near their base residues known to be essential for activity. These include Asp40 and Asn62, which function as ligands for the bound calcium ions. A pronounced bipolar charge distribution suggests that branched DNA substrates bind to the positively charged face with the scissile phosphates located near the divalent cations. A model for the complex with a four-way DNA junction is presented.
+[[Category: Escherichia virus T4]]
+[[Category: Large Structures]]
-==About this Structure==
+[[Category: Raaijmakers H]]
-EN7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EN7 OCA].
+[[Category: Suck D]]
+[[Category: Toro I]]
-==Reference==
+[[Category: Vix O]]
-X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture., Raaijmakers H, Vix O, Toro I, Golz S, Kemper B, Suck D, EMBO J. 1999 Mar 15;18(6):1447-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10075917 10075917]
-[[Category: Bacteriophage t4]]
-[[Category: Crossover junction endodeoxyribonuclease]]
-[[Category: Single protein]]
-[[Category: Raaijmakers, H.]]
-[[Category: Suck, D.]]
-[[Category: Toro, I.]]
-[[Category: Vix, O.]]
-[[Category: CA]]
-[[Category: ZN]]
-[[Category: dnase]]
-[[Category: endonuclease]]
-[[Category: holliday junction]]
-[[Category: resolvase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:57:52 2008''