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| {{STRUCTURE_2z2x| PDB=2z2x | SIZE=400| SCENE=Subtilisin/Cv/1 |right|CAPTION=Subtilisin with monoisopropylphosphorylserine complex with Ca+2 ions (green) [[2z2x]] }}
| | <StructureSection load='' size='350' side='right' caption='Subtilisin (deepskyblue) complex with streptomycin inhibitor (green) and Ca+2 ions (green) (PDB entry [[2sic]])' scene='43/430882/Cv/2'> |
| | == Function == |
| | [[Subtilisin]] is a serine protease. A 106 amino acid propeptide is cleaved from the N-terminus of '''pro-subtilisin''' to create the '''mature''' active enzym<ref>PMID:4967581</ref>. See detalis in [[User:Tommie Hata/Introduction to Protein Engineering-Subtilisin]]. |
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| [[Subtilisin]] is a serine protease. It is widely used commercially in detergents.
| | *'''Selenosubtilisin''' is a semisynthetic selenoenzyme produced by chemical modification of subtilisin<ref>PMID:8385489</ref>.<br /> |
| A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzyme. See detalis in [[User:Tommie Hata/Introduction to Protein Engineering-Subtilisin]].
| | *'''Savinase''' is a subtilisin produced from a lentil<ref>PMID:24738747</ref>.<br /> |
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| == 3D Structures of Subtilisin == | | == Relevance == |
| | Subtilisin is widely used commercially in detergents. |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | == Structural highlights == |
| | The active site of Sub contains the <scene name='43/430882/Cv/12'>catalytic triad: Ser-His-Asp</scene>. The <scene name='43/430882/Cv/13'>peptide inhibitor has numerous interactions with Sub</scene> and the <scene name='43/430882/Cv/14'>scissile bond is flanked by Cys-Pro-Met-Val</scene><ref>PMID:1920411</ref>. |
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| === Native mature subtilisin === | | == 3D Structures of Subtilisin == |
| | | [[Subtilisin 3D structures]] |
| [[2z2x]] – TkSub - ''Thermococcus kodakarensis''<br />
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| [[1sca]], [[1scb]], [[1sbc]], [[3unx]] – BsSub - ''Bacillus subtilis'' (Subtilisin Carlsberg)<br />
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| [[1pxq]] – BsSub - NMR<br />
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| [[1gns]], [[1sup]], [[1st2]], [[1s01]], [[1sbt]], [[2sbt]], [[2st1]] – BaSub - ''Bacillus amyloliquefaciens''<br />
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| [[1iav]], [[1gci]], [[1jea]], [[1st3]] – BlSub - ''Bacillus lentus'' (Subtilisin Savinase)<br />
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| [[1ea7]], [[3d43]], [[2ixt]] – spehricase – Sub from ''Bacillus sphaericus''<br />
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| [[2gko]] – Sub – psychrophilic ''Bacillus subtilis''
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| === Mutant mature subtilisin ===
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| [[3f49]], [[3co0]], [[2zrq]], [[3vhq]] – TkSub (mutant) <br /> | |
| [[1gnv]], [[1dui]], [[1sue]], [[1a2q]], [[1aqn]], [[1sua]], [[1au9]], [[1ak9]], [[1yja]], [[1yjb]], [[1yjc]], [[1sbh]], [[1sbi]], [[1sub]], [[1suc]], [[1sud]], [[1s02]] – BaSub (mutant)<br />
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| [[1ndu]], [[1q5p]], [[1c9j]], [[1c9m]], [[1c9n]] – BlSub (mutant)<br />
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| [[2xrm]] - BcSub residues 19-321 - ''Bacillus clausii''<br />
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| === Unautoprocessed subtilisin (pro-Sub) ===
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| [[2z2z]], [[2e1p]] – pro-TkSub <br />
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| [[2zwo]], [[2zwp]], [[4jp8]] – pro-TKSub (mutant)<br />
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| [[2wv7]] – pro-BcSub <br />
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| [[2wwt]], [[2x8j]] - pro-BcSub (mutant)<br />
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| [[3whi]] - pro-BcSub (mutant)<br />
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| === Mutant unautoprocessed subtilisin ===
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| [[2zwo]], [[2zwp]] – pro-TKSub
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| === Mature subtilisin complexed with its propeptide ===
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| [[3cnq]] – BaSub<br />
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| [[1spb]] – BaSub (mutant)<br />
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| [[3a3n]], [[3a3o]], [[3a3p]], [[2z56]], [[2z57]], [[2z58]], [[2z2y]], [[2z30]] – TkSub<br />
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| [[3vv2]] – TkSub (mutant)<br />
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| [[1scj]] – BsSub
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| === Subtilisin complexed with polypeptide inhibitor ===
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| [[1y1k]], [[1y33]], [[1y34]], [[1y3b]], [[1y3c]], [[1y3d]], [[1y3f]], [[1y48]], [[1y4a]], [[1y4d]], [[1tm3]], [[1tm4]],
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| [[1tm1]], [[1lw6]] – BaSub+ chymotripsin inhibitor 2<br />
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| [[1tm5]], [[1tm7]], [[1tmg]], [[1to1]], [[1to2]] - BaSub+ chymotrypsin inhibitor 2 (mutant)<br />
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| [[3sic]], [[5sic]], [[2sic]] – BaSub+Streptomyces inhibitor SSI<br />
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| [[1yu6]] – BsSub+ovomucoid <br />
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| [[1v5i]] – BaSubb+POIA1 peptide <br />
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| [[1tk2]], [[1ndq]] – BlSub+gramicidin S <br />
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| [[1r0r]] – BsSub+ovomucoid<br />
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| [[1oyv]] – BsSub+tomato inhibitor II<br />
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| [[1sbn]], [[1sib]], [[2sec]], [[1sni]], [[1cse]] – BsSub+Elgin C<br />
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| [[2sni]] - BaSub+Elgin C2 <br />
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| [[1mee]] - BpSub+Elgin C – ''Bacillus pumilus''
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| === Subtilisin complexed with inhibitor ===
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| [[3bgo]] – BaSub+azide <br />
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| [[1c3l]] – BsSub+Xe<br />
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| [[1be8]] – BsSub+cinnamoyl<br />
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| [[1be6]] - BsSub+cinnamoyl+acetonitrile<br />
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| [[1bfk]], [[1scd]] – BsSub+acetonitrile<br />
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| [[1bfu]], [[1af4]] – BsSub+dioxane<br />
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| [[1av7]], [[1avt]], [[3vsb]], [[1vsb]] – BsSub+boronic acid derivatives <br />
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| [[1scn]] – BsSub+carbamate derivative <br />
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| [[1bh6]] – Sub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone – ''Bacillus licheniformis''<br />
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| [[2wuv]], [[2wuw]] – BlSub fragment+acetonitrile
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| ===Selenosubtilisin===
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| [[1ubn]] – BaSel-Sub<br />
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| [[1sel]] – BsSel-Sub
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| ===Savinase===
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| [[3bx1]], [[1svn]], [[4cfy]], [[4cfz]], [[4cg0]] – Sav – ''Bacillus lentus''
| | </StructureSection> |
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| | == References == |
| | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |