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[[Image:1avq.gif|left|200px]]


{{Structure
==TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS==
|PDB= 1avq |SIZE=350|CAPTION= <scene name='initialview01'>1avq</scene>, resolution 2.4&Aring;
<StructureSection load='1avq' size='340' side='right'caption='[[1avq]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=ACT:ACETATE ION'>ACT</scene>
<table><tr><td colspan='2'>[[1avq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AVQ FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_(lambda-induced) Exodeoxyribonuclease (lambda-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.3 3.1.11.3]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1avq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1avq OCA], [https://pdbe.org/1avq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1avq RCSB], [https://www.ebi.ac.uk/pdbsum/1avq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1avq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EXO_LAMBD EXO_LAMBD] Facilitates phage DNA recombination through the double-strand break repair (DSBR) and single-strand annealing pathways. Also important for the late, rolling-circle mode of lambda DNA replication.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/av/1avq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1avq ConSurf].
<div style="clear:both"></div>


'''TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS'''
==See Also==
 
*[[Exonuclease 3D structures|Exonuclease 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Structure determination at 2.4 angstrom resolution shows that lambda-exonuclease consists of three subunits that form a toroid. The central channel is funnel shaped, tapering from an inner diameter of about 30 angstroms at the wider end to 15 angstroms at the narrow end. This is adequate to accommodate the DNA substrate and thus provides a structural basis for the ability of the enzyme to sequentially hydrolyze thousands of nucleotides in a highly processive manner. The results also suggest the locations of the active sites and the constraints that limit cleavage to a single strand.
[[Category: Escherichia virus Lambda]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Kovall RA]]
1AVQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVQ OCA].
[[Category: Matthews BW]]
 
==Reference==
Toroidal structure of lambda-exonuclease., Kovall R, Matthews BW, Science. 1997 Sep 19;277(5333):1824-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9295273 9295273]
[[Category: Enterobacteria phage lambda]]
[[Category: Exodeoxyribonuclease (lambda-induced)]]
[[Category: Single protein]]
[[Category: Kovall, R A.]]
[[Category: Matthews, B W.]]
[[Category: ACT]]
[[Category: PO4]]
[[Category: 5'-3' exonuclease]]
[[Category: deoxyribonuclease]]
[[Category: dna recombination and repair]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:02:50 2008''

Latest revision as of 09:33, 7 February 2024

TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMSTOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS

Structural highlights

1avq is a 3 chain structure with sequence from Escherichia virus Lambda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EXO_LAMBD Facilitates phage DNA recombination through the double-strand break repair (DSBR) and single-strand annealing pathways. Also important for the late, rolling-circle mode of lambda DNA replication.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1avq, resolution 2.40Å

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