3psh: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
==Classification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein MolA (MolA bound to Molybdate)==
==Classification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein MolA (MolA bound to Molybdate)==
<StructureSection load='3psh' size='340' side='right' caption='[[3psh]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='3psh' size='340' side='right'caption='[[3psh]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3psh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PSH FirstGlance]. <br>
<table><tr><td colspan='2'>[[3psh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PSH FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3psa|3psa]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HI1472, HI_1472 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3psh OCA], [https://pdbe.org/3psh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3psh RCSB], [https://www.ebi.ac.uk/pdbsum/3psh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3psh ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3psh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3psh RCSB], [http://www.ebi.ac.uk/pdbsum/3psh PDBsum]</span></td></tr>
</table>
<table>
== Function ==
<div style="background-color:#fffaf0;">
[https://www.uniprot.org/uniprot/MOLA_HAEIN MOLA_HAEIN] Part of the ABC transporter complex MolBCA involved in molybdate import (PubMed:22078568, PubMed:24722984). Functions as a low-affinity molybdate transporter (PubMed:24722984). Binds to both molybdate and tungstate, but not to sulfate or phosphate (PubMed:22078568).<ref>PMID:22078568</ref> <ref>PMID:24722984</ref>  
== Publication Abstract from PubMed ==
molA (HI1472) from H. influenzae encodes a periplasmic binding protein (PBP) that delivers substrate to the ABC transporter MolB(2)C(2) (formerly HI1470/71). The structures of MolA with molybdate and tungstate in the binding pocket were solved to 1.6 and 1.7 A resolution, respectively. The MolA-binding protein binds molybdate and tungstate, but not other oxyanions such as sulfate and phosphate, making it the first class III molybdate-binding protein structurally solved. The approximately 100 muM binding affinity for tungstate and molybdate is significantly lower than observed for the class II ModA molybdate-binding proteins that have nanomolar to low micromolar affinity for molybdate. The presence of two molybdate loci in H. influenzae suggests multiple transport systems for one substrate, with molABC constituting a low-affinity molybdate locus.
 
Classification of a Haemophilus influenzae ABC Transporter HI1470/71 through Its Cognate Molybdate Periplasmic Binding Protein, MolA.,Tirado-Lee L, Lee A, Rees DC, Pinkett HW Structure. 2011 Nov 9;19(11):1701-10. PMID:22078568<ref>PMID:22078568</ref>
 
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
== References ==
<references/>
<references/>
Line 21: Line 15:
</StructureSection>
</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Lee, A.]]
[[Category: Large Structures]]
[[Category: Pinkett, H W.]]
[[Category: Lee A]]
[[Category: Rees, D C.]]
[[Category: Pinkett HW]]
[[Category: Tirado-Lee, L.]]
[[Category: Rees DC]]
[[Category: Metal transport]]
[[Category: Tirado-Lee L]]
[[Category: Molybdate binding protein]]
[[Category: Periplasmic binding protein]]
[[Category: Substrate binding protein]]

Latest revision as of 13:43, 21 February 2024

Classification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein MolA (MolA bound to Molybdate)Classification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein MolA (MolA bound to Molybdate)

Structural highlights

3psh is a 1 chain structure with sequence from Haemophilus influenzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MOLA_HAEIN Part of the ABC transporter complex MolBCA involved in molybdate import (PubMed:22078568, PubMed:24722984). Functions as a low-affinity molybdate transporter (PubMed:24722984). Binds to both molybdate and tungstate, but not to sulfate or phosphate (PubMed:22078568).[1] [2]

References

  1. ↑ Tirado-Lee L, Lee A, Rees DC, Pinkett HW. Classification of a Haemophilus influenzae ABC Transporter HI1470/71 through Its Cognate Molybdate Periplasmic Binding Protein, MolA. Structure. 2011 Nov 9;19(11):1701-10. PMID:22078568 doi:10.1016/j.str.2011.10.004
  2. ↑ Rice AJ, Harrison A, Alvarez FJ, Davidson AL, Pinkett HW. Small substrate transport and mechanism of a molybdate ATP binding cassette transporter in a lipid environment. J Biol Chem. 2014 May 23;289(21):15005-13. PMID:24722984 doi:10.1074/jbc.M114.563783

3psh, resolution 1.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3psh&oldid=4067454"