2mpk: Difference between revisions
New page: '''Unreleased structure''' The entry 2mpk is ON HOLD Authors: Bulone, V., Szpryngiel, S., Brown, C., Ye, W., , . Description: Characterization and structure of the MIT1 domain of a chi... |
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==Characterization and structure of the MIT1 domain of a chitin synthase from the Oomycete Saprolegnia monoica== | |||
<StructureSection load='2mpk' size='340' side='right'caption='[[2mpk]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2mpk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saprolegnia_monoica Saprolegnia monoica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MPK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mpk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mpk OCA], [https://pdbe.org/2mpk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mpk RCSB], [https://www.ebi.ac.uk/pdbsum/2mpk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mpk ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/D7NQS8_SAPMO D7NQS8_SAPMO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Chitin synthases (Chs) are responsible for the synthesis of chitin, a key structural cell wall polysaccharide in many organisms. They are essential for growth in certain oomycete species, some of which are pathogenic to diverse higher organisms. Recently, a microtubule interacting and trafficking (MIT) domain, which is not found in any fungal Chs, has been identified in some oomycete Chs proteins. Based on experimental data relating to the binding specificity of other eukaryotic MIT domains, there was speculation that this domain may be involved in the intracellular trafficking of Chs proteins. However, there is currently no evidence for this or any other function for the MIT domain in these enzymes. To attempt to elucidate their function, MIT domains from two Chs enzymes from the oomycete Saprolegnia monoica were cloned, expressed, and characterized. Both were shown to interact strongly with the plasma membrane component, phosphatidic acid, and to have additional putative interactions with proteins thought to be involved in protein transport and localization. Aiding our understanding of these data, the structure of the first MIT domain from a carbohydrate-active enzyme (MIT1) was solved by NMR, and a model structure of a second MIT domain (MIT2) was built by homology modeling. Our results suggest a potential function for these MIT domains in the intracellular transport and/or regulation of Chs enzymes in the oomycetes. DATABASE: Structural data are available in the Biological Magnetic Resonance Bank (BMRB) database under the accession number 19987 and the PDB database under the accession number 2MPK. | |||
Structural and functional characterization of the microtubule interacting and trafficking domains of two oomycete chitin synthases.,Brown C, Szpryngiel S, Kuang G, Srivastava V, Ye W, McKee LS, Tu Y, Maler L, Bulone V FEBS J. 2016 Aug;283(16):3072-88. doi: 10.1111/febs.13794. Epub 2016 Jul 22. PMID:27363606<ref>PMID:27363606</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2mpk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saprolegnia monoica]] | |||
[[Category: Brown C]] | |||
[[Category: Bulone V]] | |||
[[Category: Szpryngiel S]] | |||
[[Category: Ye W]] | |||