1aip: Difference between revisions

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-[[Image:1aip.gif|left|200px]]
- {{Structure
+==EF-TU EF-TS COMPLEX FROM THERMUS THERMOPHILUS==
-|PDB= 1aip |SIZE=350|CAPTION= <scene name='initialview01'>1aip</scene>, resolution 3.0&Aring;
+<StructureSection load='1aip' size='340' side='right'caption='[[1aip]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[1aip]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AIP FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
-|GENE=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aip OCA], [https://pdbe.org/1aip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aip RCSB], [https://www.ebi.ac.uk/pdbsum/1aip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aip ProSAT]</span></td></tr>
-}}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/EFTU1_THETH EFTU1_THETH] This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/1aip_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aip ConSurf].
+<div style="clear:both"></div>
-'''EF-TU EF-TS COMPLEX FROM THERMUS THERMOPHILUS'''
+==See Also==
+*[[Elongation factor 3D structures|Elongation factor 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-In order to study nucleotide exchange mechanisms in GTP-binding proteins, we have determined the crystal structure of the complex formed by the elongation factor Tu (EF-Tu) and its exchange factor Ts (EF-Ts) from Thermus thermophilus. The complex is a dyad symmetrical heterotetramer in which each EF-Tu, through a bipartite interface, interacts with two subunits of EF-Ts, explaining the need for a dimeric exchange factor. The architecture of the assembly is distinctly different from that of the corresponding heterodimeric E. coli complex, in which the monomeric E. coli EF-Ts remarkably forms essentially the same bipartite interface with EF-Tu through a sequence/structural repeat. GDP is released primarily by a Ts-induced peptide flip in the nucleotide binding pocket that disrupts hydrogen bonds to the phosphates and repositions the peptide carbonyl so as to sterically and electrostatically eject the GDP. The exchange mechanism may have useful implications for receptor-induced exchange in heterotrimeric G proteins.
+[[Category: Large Structures]]
-==About this Structure==
-AIP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIP OCA].
-==Reference==
-Crystal structure of the EF-Tu.EF-Ts complex from Thermus thermophilus., Wang Y, Jiang Y, Meyering-Voss M, Sprinzl M, Sigler PB, Nat Struct Biol. 1997 Aug;4(8):650-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9253415 9253415]
-[[Category: Protein complex]]
 [[Category: Thermus thermophilus]]
-[[Category: Jiang, Y.]]
+[[Category: Jiang Y]]
-[[Category: Meyering-Voss, M.]]
+[[Category: Meyering-Voss M]]
-[[Category: Sigler, P B.]]
+[[Category: Sigler PB]]
-[[Category: Sprinzl, M.]]
+[[Category: Sprinzl M]]
-[[Category: Wang, Y.]]
+[[Category: Wang Y]]
-[[Category: complex of two elongation factor]]
-[[Category: elongation factor]]
-[[Category: gtp-binding]]
-[[Category: nucleotide exchange]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:58:06 2008''