1a8z: Difference between revisions

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[[Image:1a8z.gif|left|200px]]


{{Structure
==STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS==
|PDB= 1a8z |SIZE=350|CAPTION= <scene name='initialview01'>1a8z</scene>, resolution 2.1&Aring;
<StructureSection load='1a8z' size='340' side='right'caption='[[1a8z]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CU1:COPPER (I) ION'>CU1</scene>
<table><tr><td colspan='2'>[[1a8z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A8Z FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a8z OCA], [https://pdbe.org/1a8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a8z RCSB], [https://www.ebi.ac.uk/pdbsum/1a8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a8z ProSAT]</span></td></tr>
 
</table>
'''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS'''
== Function ==
 
[https://www.uniprot.org/uniprot/RUS2_ACIFI RUS2_ACIFI] Electron carrier from cytochrome c552 to the A-type oxidase.
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The structure of rusticyanin, an acid-stable copper protein, has been determined at 2.1 A resolution by direct methods combined with the single-wavelength anomalous scattering (SAS) of copper (f" = 3.9 e-) and then conventionally refined (Rcryst = 18.7%, Rfree = 21.9%). This is the largest unknown protein structure (Mr approximately /= 16.8 kDa) to be determined using the SAS and direct-methods approach and demonstrates that by exploiting the anomalous signal at a single wavelength, direct methods can be used to determine phases at typical (approximately 2 A) macromolecular crystallographic resolutions. Extrapolating from the size of the anomalous signal for copper (f" approximately 4 e-), this result suggests that the approach could be used for proteins with molecular weights of up to 33 kDa per Se (f"max++ = 8 e- at the 'white line') and 80 kDa for a Pt derivative (f"max = 19 e- at the 'white line', L3 edge). The method provides a powerful alternative in solving a de novo protein structure without either preparing multiple crystals (i.e. isomorphous heavy-atom derivative plus native crystals) or collecting multi-wavelength anomalous diffraction (MAD) data.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/1a8z_consurf.spt"</scriptWhenChecked>
1A8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8Z OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9761859 9761859]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a8z ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Acidithiobacillus ferrooxidans]]
[[Category: Acidithiobacillus ferrooxidans]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Duke, E M.H.]]
[[Category: Duke EMH]]
[[Category: Hao, Q.]]
[[Category: Hao Q]]
[[Category: Harvey, I.]]
[[Category: Harvey I]]
[[Category: Hasnain, S S.]]
[[Category: Hasnain SS]]
[[Category: Ingledew, W J.]]
[[Category: Ingledew WJ]]
[[Category: CU1]]
[[Category: copper protein]]
[[Category: direct method]]
[[Category: electron transport]]
[[Category: medium resolution]]
[[Category: metalloprotein]]
[[Category: sa]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:54:34 2008''

Latest revision as of 09:28, 7 February 2024

STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODSSTRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS

Structural highlights

1a8z is a 1 chain structure with sequence from Acidithiobacillus ferrooxidans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RUS2_ACIFI Electron carrier from cytochrome c552 to the A-type oxidase.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1a8z, resolution 2.10Å

Drag the structure with the mouse to rotate

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