1a0a: Difference between revisions

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-[[Image:1a0a.gif|left|200px]]
- {{Structure
+==PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX==
-|PDB= 1a0a |SIZE=350|CAPTION= <scene name='initialview01'>1a0a</scene>, resolution 2.800&Aring;
+<StructureSection load='1a0a' size='340' side='right'caption='[[1a0a]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[1a0a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A0A FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-|GENE=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a0a OCA], [https://pdbe.org/1a0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a0a RCSB], [https://www.ebi.ac.uk/pdbsum/1a0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a0a ProSAT]</span></td></tr>
-}}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PHO4_YEAST PHO4_YEAST] Transcriptional activator that regulates the expression of repressible phosphatase under phosphate starvation conditions. Binds to the upstream activating sequence (UAS) of several phosphatase encoding PHO genes. Inhibited by the cyclin-CDK PHO80-PHO85 under high-phosphate conditions.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a0/1a0a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a0a ConSurf].
+<div style="clear:both"></div>
-'''PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX'''
+==See Also==
+*[[Pho4|Pho4]]
+__TOC__
-==Overview==
+</StructureSection>
-The crystal structure of a DNA-binding domain of PHO4 complexed with DNA at 2.8 A resolution revealed that the domain folds into a basic-helix-loop-helix (bHLH) motif with a long but compact loop that contains a short alpha-helical segment. This helical structure positions a tryptophan residue into an aromatic cluster so as to make the loop compact. PHO4 binds to DNA as a homodimer with direct reading of both the core E-box sequence CACGTG and its 3'-flanking bases. The 3'-flanking bases GG are recognized by Arg2 and His5. The residues involved in the E-box recognition are His5, Glu9 and Arg13, as already reported for bHLH/Zip proteins MAX and USF, and are different from those recognized by bHLH proteins MyoD and E47, although PHO4 is a bHLH protein.
+[[Category: Large Structures]]
-==About this Structure==
-A0A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0A OCA].
-==Reference==
-Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition., Shimizu T, Toumoto A, Ihara K, Shimizu M, Kyogoku Y, Ogawa N, Oshima Y, Hakoshima T, EMBO J. 1997 Aug 1;16(15):4689-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9303313 9303313]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Hakoshima T]]
-[[Category: Hakoshima, T.]]
+[[Category: Ihara K]]
-[[Category: Ihara, K.]]
+[[Category: Kyogoku Y]]
-[[Category: Kyogoku, Y.]]
+[[Category: Ogawa N]]
-[[Category: Ogawa, N.]]
+[[Category: Oshima Y]]
-[[Category: Oshima, Y.]]
+[[Category: Shimizu M]]
-[[Category: Shimizu, M.]]
+[[Category: Shimizu T]]
-[[Category: Shimizu, T.]]
+[[Category: Toumoto A]]
-[[Category: Toumoto, A.]]
-[[Category: basic helix loop helix]]
-[[Category: complex (transcription factor/dna)]]
-[[Category: transcription factor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:51:08 2008''