4bpy: Difference between revisions

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-{{STRUCTURE_4bpy|  PDB=4bpy  |  SCENE=  }}
-===Crystal structure of the C90A mutant of the Sco copper chaperone protein from Streptomyces lividans===
-{{ABSTRACT_PUBMED_24548299}}
-==About this Structure==
+==Crystal structure of the C90A mutant of the Sco copper chaperone protein from Streptomyces lividans==
-[[4bpy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_lividans"_krasil'nikov_et_al._1965 "actinomyces lividans" krasil'nikov et al. 1965]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPY OCA].
+<StructureSection load='4bpy' size='340' side='right'caption='[[4bpy]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4bpy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BPY FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bpy OCA], [https://pdbe.org/4bpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bpy RCSB], [https://www.ebi.ac.uk/pdbsum/4bpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bpy ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q93J40_STRCO Q93J40_STRCO]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In Streptomyces lividans an extracytoplasmic copper-binding Sco protein plays a role in two unlinked processes: (i) initiating a morphological development switch and (ii) facilitating the co-factoring of the CuA domain of CcO (cytochrome c oxidase). How Sco obtains copper once secreted to the extracytoplasmic environment is unknown. In the present paper we report on a protein possessing an HX6MX21HXM motif that binds a single cuprous ion with subfemtomolar affinity. High-resolution X-ray structures of this extracytoplasmic copper chaperone-like protein (ECuC) in the apo- and Cu(I)-bound states reveal that the latter possesses a surface-accessible cuprous-ion-binding site located in a dish-shaped region of beta-sheet structure. A cuprous ion is transferred under a favourable thermodynamic gradient from ECuC to Sco with no back transfer occurring. The ionization properties of the cysteine residues in the Cys86xxxCys90 copper-binding motif of Sco, together with their positional locations identified from an X-ray structure of Sco, suggests a role for Cys86 in initiating an inter-complex ligand-exchange reaction with Cu(I)-ECuC. Generation of the genetic knockouts, Deltasco, Deltaecuc and Deltasco/ecuc, and subsequent in vivo assays lend support to the existence of a branched extracytoplasmic copper-trafficking pathway in S. lividans. One branch requires both Sco and to a certain extent ECuC to cofactor the CuA domain, whereas the other uses only Sco to deliver copper to a cuproenzyme to initiate morphological development.
-==Reference==
+Structural and mechanistic insights into an extracytoplasmic copper trafficking pathway in Streptomyces lividans.,Blundell KL, Hough MA, Vijgenboom E, Worrall JA Biochem J. 2014 May 1;459(3):525-38. doi: 10.1042/BJ20140017. PMID:24548299<ref>PMID:24548299</ref>
-<ref group="xtra">PMID:024548299</ref><references group="xtra"/><references/>
-[[Category: Actinomyces lividans krasil'nikov et al. 1965]]
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Blundell, K L.I M.]]
+</div>
-[[Category: Hough, M A.]]
+<div class="pdbe-citations 4bpy" style="background-color:#fffaf0;"></div>
-[[Category: Worrall, J A.R.]]
+== References ==
-[[Category: Chaperone]]
+<references/>
-[[Category: Copper homeostasis]]
+__TOC__
-[[Category: Homeostasis]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptomyces lividans]]
+[[Category: Blundell KLIM]]
+[[Category: Hough MA]]
+[[Category: Worrall JAR]]