4p70: Difference between revisions
New page: '''Unreleased structure''' The entry 4p70 is ON HOLD Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M. Description: Crystal Structure of Unmodified tRNA Proline (CGG) Bound to Codon ... |
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==Crystal Structure of Unmodified tRNA Proline (CGG) Bound to Codon CCG on the Ribosome== | |||
<StructureSection load='4p70' size='340' side='right'caption='[[4p70]], [[Resolution|resolution]] 3.68Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4p70]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vy0 1vy0], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vy1 1vy1], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vy2 1vy2] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vy3 1vy3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P70 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.68Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p70 OCA], [https://pdbe.org/4p70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p70 RCSB], [https://www.ebi.ac.uk/pdbsum/4p70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p70 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RS3_THET8 RS3_THET8] Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.[HAMAP-Rule:MF_01309_B] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6 and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame. | |||
Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388<ref>PMID:25128388</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4p70" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]] | |||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Thermus thermophilus HB8]] | |||
[[Category: Dunham CM]] | |||
[[Category: Dunkle JA]] | |||
[[Category: Maehigashi T]] | |||