4lhx: Difference between revisions

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-{{STRUCTURE_4lhx|  PDB=4lhx  |  SCENE=  }}
-===Crystal structure of nucleotide-free Rab8:Rabin8===
-{{ABSTRACT_PUBMED_24072714}}
-==Function==
+==Crystal structure of nucleotide-free Rab8:Rabin8==
-[[http://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN]] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>  [[http://www.uniprot.org/uniprot/RAB3I_HUMAN RAB3I_HUMAN]] Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.<ref>PMID:12221131</ref> <ref>PMID:20937701</ref> <ref>PMID:20890297</ref>
+<StructureSection load='4lhx' size='340' side='right'caption='[[4lhx]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4lhx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LHX FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhx OCA], [https://pdbe.org/4lhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lhx RCSB], [https://www.ebi.ac.uk/pdbsum/4lhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lhx ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Small G-proteins (G) of the Ras-superfamily control the temporal and spatial coordination of intracellular signaling networks by acting as molecular on/off switches. Guanine nucleotide exchange factors (GEFs) regulate the activation of these G-proteins through catalytic replace-ment of guanosine diphosphate (GDP) by gua-nosine triphosphate (GTP). During nucleotide exchange, three distinct substrate-enzyme complexes occur: A ternary complex with GDP at the start of reaction (G:GDP:GEF), an intermediary nucleotide-free binary complex (G:GEF), and a ternary GTP-complex after productive G-protein activation (G:GTP:GEF). Here we show structural snapshots of the full nucleotide-exchange reaction sequence together with the G-protein substrates and products using Rabin8/GRAB (GEF) and Rab8 (G-protein) as a model system. Together with a thorough enzymatic characterization, our data provide a detailed view into the mechanism of Rabin8/GRAB mediated nucleotide exchange.
-==About this Structure==
+Intermediates in the Guanine Nucleotide Exchange Reaction of Rab8 Catalyzed by Rabin8/GRAB.,Guo Z, Hou X, Goody RS, Itzen A J Biol Chem. 2013 Sep 26. PMID:24072714<ref>PMID:24072714</ref>
-[[4lhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHX OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:024072714</ref><references group="xtra"/><references/>
+</div>
-[[Category: Human]]
+<div class="pdbe-citations 4lhx" style="background-color:#fffaf0;"></div>
-[[Category: Goody, R S.]]
+== References ==
-[[Category: Guo, Z.]]
+<references/>
-[[Category: Hou, X M.]]
+__TOC__
-[[Category: Itzen, A.]]
+</StructureSection>
-[[Category: Guanine nucleotide exchange factor]]
+[[Category: Homo sapiens]]
-[[Category: Protein transport]]
+[[Category: Large Structures]]
-[[Category: Small gtpase]]
+[[Category: Goody RS]]
+[[Category: Guo Z]]
+[[Category: Hou XM]]
+[[Category: Itzen A]]