4c7o: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4c7o is ON HOLD
+==The structural basis of FtsY recruitment and GTPase activation by SRP RNA==
+<StructureSection load='4c7o' size='340' side='right'caption='[[4c7o]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4c7o]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C7O FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c7o OCA], [https://pdbe.org/4c7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c7o RCSB], [https://www.ebi.ac.uk/pdbsum/4c7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c7o ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SRP54_ECOLI SRP54_ECOLI] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.<ref>PMID:2171778</ref> <ref>PMID:1279430</ref> <ref>PMID:1331806</ref> <ref>PMID:9305630</ref> <ref>PMID:11735405</ref> <ref>PMID:11741850</ref> <ref>PMID:15140892</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The universally conserved signal recognition particle (SRP) system mediates the targeting of membrane proteins to the translocon in a multistep process controlled by GTP hydrolysis. Here we present the 2.6 A crystal structure of the GTPase domains of the E. coli SRP protein (Ffh) and its receptor (FtsY) in complex with the tetraloop and the distal region of SRP-RNA, trapped in the activated state in presence of GDP:AlF4. The structure reveals the atomic details of FtsY recruitment and, together with biochemical experiments, pinpoints G83 as the key RNA residue that stimulates GTP hydrolysis. Insertion of G83 into the FtsY active site orients a single glutamate residue provided by Ffh (E277), triggering GTP hydrolysis and complex disassembly at the end of the targeting cycle. The complete conservation of the key residues of the SRP-RNA and the SRP protein implies that the suggested chemical mechanism of GTPase activation is applicable across all kingdoms.
-Authors: Voigts-Hoffmann, F., Schmitz, N., Shen, K., Shan, S.O., Ataide, S.F., Ban, N.
+The Structural Basis of FtsY Recruitment and GTPase Activation by SRP RNA.,Voigts-Hoffmann F, Schmitz N, Shen K, Shan SO, Ataide SF, Ban N Mol Cell. 2013 Nov 6. pii: S1097-2765(13)00746-6. doi:, 10.1016/j.molcel.2013.10.005. PMID:24211265<ref>PMID:24211265</ref>
-Description: The structural basis of FtsY recruitment and GTPase activation by SRP RNA
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4c7o" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]]
+*[[Signal recognition particle receptor 3D structures|Signal recognition particle receptor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli K-12]]
+[[Category: Large Structures]]
+[[Category: Ataide SF]]
+[[Category: Ban N]]
+[[Category: Schmitz N]]
+[[Category: Shan SO]]
+[[Category: Shen K]]
+[[Category: Voigts-Hoffmann F]]