2np2: Difference between revisions

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-[[Image:2np2.gif|left|200px]]<br /><applet load="2np2" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2np2, resolution 3.02&Aring;" />
-'''Hbb-DNA complex'''<br />
-==Overview==
+==Hbb-DNA complex==
+<StructureSection load='2np2' size='340' side='right'caption='[[2np2]], [[Resolution|resolution]] 3.02&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2np2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi Borreliella burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NP2 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.02&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2np2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2np2 OCA], [https://pdbe.org/2np2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2np2 RCSB], [https://www.ebi.ac.uk/pdbsum/2np2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2np2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DBH_BORBU DBH_BORBU] Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2np2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2np2 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
 The genome of the Lyme disease-causing spirochete Borrelia burgdorferi encodes only a single polypeptide from the integration host factor (IHF)/HU or 'DNABII' family of nucleoid-associated proteins - Hbb. DNABII proteins induce large bends in DNA and serve as architectural factors in a variety of prokaryotic cellular processes. We have solved the crystal structure of an Hbb-DNA complex in which the DNA is bent by over 180 degrees . We find that like IHF, Hbb relies exclusively on indirect readout to recognize its cognate site. Additional binding studies show that the sequence preferences of Hbb are related to, yet distinct from those of IHF. Defining these binding characteristics may help to uncover additional roles for Hbb in Borrelia DNA metabolism as well as further our understanding of the mechanism of indirect readout.
-==About this Structure==
+Shaping the Borrelia burgdorferi genome: crystal structure and binding properties of the DNA-bending protein Hbb.,Mouw KW, Rice PA Mol Microbiol. 2007 Mar;63(5):1319-30. Epub 2007 Jan 22. PMID:17244195<ref>PMID:17244195</ref>
-NP2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP2 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Shaping the Borrelia burgdorferi genome: crystal structure and binding properties of the DNA-bending protein Hbb., Mouw KW, Rice PA, Mol Microbiol. 2007 Mar;63(5):1319-30. Epub 2007 Jan 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17244195 17244195]
+</div>
-[[Category: Borrelia burgdorferi]]
+<div class="pdbe-citations 2np2" style="background-color:#fffaf0;"></div>
-[[Category: Single protein]]
+== References ==
-[[Category: Mouw, K W.]]
+<references/>
-[[Category: Rice, P A.]]
+__TOC__
-[[Category: dna-bending protein]]
+</StructureSection>
-[[Category: dna-binding protein]]
+[[Category: Borreliella burgdorferi]]
-[[Category: dnabii family]]
+[[Category: Large Structures]]
-[[Category: hu/ihf family]]
+[[Category: Mouw KW]]
-[[Category: protein-dna complex]]
+[[Category: Rice PA]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:09:30 2008''