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{{STRUCTURE_4l6y|  PDB=4l6y  |  SCENE=  }}
===Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)===
{{ABSTRACT_PUBMED_23870126}}


==Function==
==Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)==
[[http://www.uniprot.org/uniprot/PRC1_HUMAN PRC1_HUMAN]] Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle.<ref>PMID:9885575</ref> <ref>PMID:12082078</ref> <ref>PMID:15297875</ref> <ref>PMID:15625105</ref> <ref>PMID:16431929</ref> <ref>PMID:19468300</ref> <ref>PMID:20691902</ref>
<StructureSection load='4l6y' size='340' side='right'caption='[[4l6y]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4l6y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L6Y FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3015&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6y OCA], [https://pdbe.org/4l6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l6y RCSB], [https://www.ebi.ac.uk/pdbsum/4l6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l6y ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRC1_HUMAN PRC1_HUMAN] Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle.<ref>PMID:9885575</ref> <ref>PMID:12082078</ref> <ref>PMID:15297875</ref> <ref>PMID:15625105</ref> <ref>PMID:16431929</ref> <ref>PMID:19468300</ref> <ref>PMID:20691902</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Error-free cell division depends on the assembly of the spindle midzone, a specialized array of overlapping microtubules that emerges between segregating chromosomes during anaphase. The molecular mechanisms by which a subset of dynamic microtubules from the metaphase spindle are selected and organized into a stable midzone array are poorly understood. Here, we show using in vitro reconstitution assays that PRC1 and kinesin-4, two microtubule-associated proteins required for midzone assembly, can tag microtubule plus ends. Remarkably, the size of these tags is proportional to filament length. We determine the crystal structure of the PRC1 homodimer and map the protein-protein interactions needed for tagging microtubule ends. Importantly, length-dependent microtubule plus-end-tagging by PRC1 is also observed in dividing cells. Our findings suggest how biochemically similar microtubules can be differentially marked, based on length, for selective regulation during the formation of specialized arrays, such as those required for cytokinesis.


==About this Structure==
Marking and Measuring Single Microtubules by PRC1 and Kinesin-4.,Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM Cell. 2013 Jul 18;154(2):377-90. doi: 10.1016/j.cell.2013.06.021. PMID:23870126<ref>PMID:23870126</ref>
[[4l6y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6Y OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023870126</ref><references group="xtra"/><references/>
</div>
<div class="pdbe-citations 4l6y" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Darst, S A.]]
[[Category: Large Structures]]
[[Category: Kapoor, T M.]]
[[Category: Darst SA]]
[[Category: Subramanian, R.]]
[[Category: Kapoor TM]]
[[Category: Tan, L.]]
[[Category: Subramanian R]]
[[Category: Ti, S.]]
[[Category: Tan L]]
[[Category: Kinesin-4]]
[[Category: Ti S]]
[[Category: Microtubule]]
[[Category: Microtubule binding]]
[[Category: Microtubule crosslinking]]
[[Category: Spectrin]]
[[Category: Spindle midzone]]
[[Category: Structural protein]]

Latest revision as of 19:13, 20 September 2023

Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)

Structural highlights

4l6y is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.3015Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRC1_HUMAN Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle.[1] [2] [3] [4] [5] [6] [7]

Publication Abstract from PubMed

Error-free cell division depends on the assembly of the spindle midzone, a specialized array of overlapping microtubules that emerges between segregating chromosomes during anaphase. The molecular mechanisms by which a subset of dynamic microtubules from the metaphase spindle are selected and organized into a stable midzone array are poorly understood. Here, we show using in vitro reconstitution assays that PRC1 and kinesin-4, two microtubule-associated proteins required for midzone assembly, can tag microtubule plus ends. Remarkably, the size of these tags is proportional to filament length. We determine the crystal structure of the PRC1 homodimer and map the protein-protein interactions needed for tagging microtubule ends. Importantly, length-dependent microtubule plus-end-tagging by PRC1 is also observed in dividing cells. Our findings suggest how biochemically similar microtubules can be differentially marked, based on length, for selective regulation during the formation of specialized arrays, such as those required for cytokinesis.

Marking and Measuring Single Microtubules by PRC1 and Kinesin-4.,Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM Cell. 2013 Jul 18;154(2):377-90. doi: 10.1016/j.cell.2013.06.021. PMID:23870126[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Jiang W, Jimenez G, Wells NJ, Hope TJ, Wahl GM, Hunter T, Fukunaga R. PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis. Mol Cell. 1998 Dec;2(6):877-85. PMID:9885575
  2. ↑ Mollinari C, Kleman JP, Jiang W, Schoehn G, Hunter T, Margolis RL. PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone. J Cell Biol. 2002 Jun 24;157(7):1175-86. Epub 2002 Jun 24. PMID:12082078 doi:10.1083/jcb.200111052
  3. ↑ Kurasawa Y, Earnshaw WC, Mochizuki Y, Dohmae N, Todokoro K. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 2004 Aug 18;23(16):3237-48. Epub 2004 Aug 5. PMID:15297875 doi:10.1038/sj.emboj.7600347
  4. ↑ Zhu C, Jiang W. Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis. Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):343-8. Epub 2004 Dec 29. PMID:15625105 doi:0408438102
  5. ↑ Gruneberg U, Neef R, Li X, Chan EH, Chalamalasetty RB, Nigg EA, Barr FA. KIF14 and citron kinase act together to promote efficient cytokinesis. J Cell Biol. 2006 Jan 30;172(3):363-72. Epub 2006 Jan 23. PMID:16431929 doi:jcb.200511061
  6. ↑ Wolfe BA, Takaki T, Petronczki M, Glotzer M. Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation. PLoS Biol. 2009 May 5;7(5):e1000110. doi: 10.1371/journal.pbio.1000110. Epub 2009, May 26. PMID:19468300 doi:10.1371/journal.pbio.1000110
  7. ↑ Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM. Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein. Cell. 2010 Aug 6;142(3):433-43. PMID:20691902 doi:10.1016/j.cell.2010.07.012
  8. ↑ Subramanian R, Ti SC, Tan L, Darst SA, Kapoor TM. Marking and Measuring Single Microtubules by PRC1 and Kinesin-4. Cell. 2013 Jul 18;154(2):377-90. doi: 10.1016/j.cell.2013.06.021. PMID:23870126 doi:10.1016/j.cell.2013.06.021

4l6y, resolution 3.30Å

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