Purine repressor: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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-<StructureSection load='1qp4' size='350' side='right' caption='Structure of purine processor complex with DNA and hypoxanthine (PDB entry [[1qp4]])' scene=''>
+<StructureSection load='' size='450' side='right' caption='Structure of E. coli purine processor complex with DNA and guanine (PDB entry [[1wet]])' scene='55/554904/Cv/1'>
+== Function ==
+'''Purine repressor''' (PurR) is a member of the lac repressor family.  PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD).  PurP contains a nucleotide co-repressor binding domain as well (CBD).  PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides<ref>PMID:1971621</ref>.
-'''Purine repressor''' (PurR) is a member of the lac repressor family.  PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD).  PurP contains a co-repressor binding domain as well (CBD).  PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides.
+== Structural highlights ==
+The <scene name='55/554904/Cv/5'>PurP guanine co-repressor binding site includes stacking interactions as well as hydrogen bonded ones</scene>. Water molecules are shown as red spheres. The DNA binding domain contains a <scene name='55/554904/Cv/6'>helix-turn-helix-loop-helix motif which interacts with the DNA major groove</scene> and a hinge helix binding to to the DNA minor groove. <scene name='55/554904/Cv/7'>Residue L54 interdigitates with the DNA central base pair</scene><ref>PMID:9278422</ref>.
+</StructureSection>
 ==3D structures of purine repressor==
 Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
-[[1pru]], [[1prv]] - EcPurR DBD – ''Escherichia coli'' - NMR<br />
+*Purine repressor
-[[1dbq]] - EcPurR CBD + Mg - NMR<br />
-[[1jhz]] - EcPurR CBD (mutant) + Mg <br />
-'''PurP complex with DNA and nucleotide'''
+**[[1pru]], [[1prv]] - EcPurR DBD – ''Escherichia coli'' - NMR<br />
+**[[1dbq]] - EcPurR CBD + Mg - NMR<br />
+**[[1jhz]] - EcPurR CBD (mutant) + Mg <br />
+**[[1o57]] - BsPurR - ''Bacillus subtilis''<br />
-[[1pnr]], [[1bdi]], [[1qp0]], [[1qp4]], [[1qpz]], [[1qqb]] - EcPurR + DNA + hypoxanthine <br />
+*Purine repressor complex with DNA and nucleotide
-[[1bdh]], [[2pud]], [[2pug]], [[1vpw]], [[1qp7]], [[1qqa]], [[1jfs]], [[1jft]], [[1jh9]] - EcPurR (mutant) + DNA + hypoxanthine <br />
-[[1wet]] - EcPurR + DNA + guanine <br />
-[[2pua]] - EcPurR (mutant) + DNA + methylpurine <br />
-[[2pub]], [[2pue]] - EcPurR (mutant) + DNA + adenine <br />
-[[2puc]], [[2puf]] - EcPurR (mutant) + DNA + guanine <br />
-[[1zay]] - EcPurR + DNA + hypoxanthine + di-imino-purine<br />
+**[[1pnr]], [[1bdi]], [[1qp0]], [[1qp4]], [[1qpz]], [[1qqb]] - EcPurR + DNA + hypoxanthine <br />
+**[[1bdh]], [[2pud]], [[2pug]], [[1vpw]], [[1qp7]], [[1qqa]], [[1jfs]], [[1jft]], [[1jh9]] - EcPurR (mutant) + DNA + hypoxanthine <br />
+**[[1wet]] - EcPurR + DNA + guanine <br />
+**[[2pua]] - EcPurR (mutant) + DNA + methylpurine <br />
+**[[2pub]], [[2pue]] - EcPurR (mutant) + DNA + adenine <br />
+**[[2puc]], [[2puf]] - EcPurR (mutant) + DNA + guanine <br />
+**[[1zay]] - EcPurR + DNA + hypoxanthine + di-imino-purine<br />
+**[[7rmw]] – BsPurR + PPGPP<br />
+}}
+== References ==
+<references/>
 [[Category:Topic Page]]