1uzv: Difference between revisions

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-[[Image:1uzv.gif|left|200px]]<br />
-<applet load="1uzv" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1uzv, resolution 1.00&Aring;" />
-'''HIGH AFFINITY FUCOSE BINDING OF PSEUDOMONAS AERUGINOSA LECTIN II: 1.0 A CRYSTAL STRUCTURE OF THE COMPLEX'''<br />
-==Overview==
+==High affinity fucose binding of Pseudomonas aeruginosa lectin II: 1.0 A crystal structure of the complex==
-PA-IIL is a fucose-binding lectin from Pseudomonas aeruginosa that is, closely related to the virulence factors of the bacterium. Previous, structural studies have revealed a new carbohydrate-binding mode with, direct involvement of two calcium ions (Mitchell E, Houles C, Sudakevitz, D, Wimmerova M, Gautier C, Perez S, Wu AM, Gilboa-Garber N, Imberty A., Structural basis for selective recognition of oligosaccharides from cystic, fibrosis patients by the lectin PA-IIL of Pseudomonas aeruginosa. Nat, Struct Biol 2002;9:918-921). A combination of thermodynamic, structural, and computational methods has been used to study the basis of the high, affinity for the monosaccharide ligand. A titration microcalorimetry study, indicated that the high affinity is enthalpy driven. The crystal ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15573375 (full description)]]
+<StructureSection load='1uzv' size='340' side='right'caption='[[1uzv]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1uzv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UZV FirstGlance]. <br>
-UZV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with FUC, SO4 and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: C1A. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UZV OCA]].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uzv OCA], [https://pdbe.org/1uzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uzv RCSB], [https://www.ebi.ac.uk/pdbsum/1uzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uzv ProSAT]</span></td></tr>
-High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches., Mitchell EP, Sabin C, Snajdrova L, Pokorna M, Perret S, Gautier C, Hofr C, Gilboa-Garber N, Koca J, Wimmerova M, Imberty A, Proteins. 2005 Feb 15;58(3):735-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15573375 15573375]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9HYN5_PSEAE Q9HYN5_PSEAE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uz/1uzv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uzv ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Pseudomonas aeruginosa]]
-[[Category: Single protein]]
+[[Category: Budova M]]
-[[Category: Budova, M.]]
+[[Category: Gautier C]]
-[[Category: Gautier, C.]]
+[[Category: Gilboa-Garber N]]
-[[Category: Gilboa-Garber, N.]]
+[[Category: Imberty A]]
-[[Category: Imberty, A.]]
+[[Category: Koca J]]
-[[Category: Koca, J.]]
+[[Category: Mitchell E]]
-[[Category: Mitchell, E.]]
+[[Category: Perret S]]
-[[Category: Perret, S.]]
+[[Category: Sabin CD]]
-[[Category: Sabin, C.D.]]
+[[Category: Snajdrova L]]
-[[Category: Snajdrova, L.]]
+[[Category: Wimmerova M]]
-[[Category: Wimmerova, M.]]
-[[Category: CA]]
-[[Category: FUC]]
-[[Category: SO4]]
-[[Category: calcium]]
-[[Category: fucose]]
-[[Category: lectin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:16:52 2007''