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[[Image:2hhc.jpg|left|200px]]<br /><applet load="2hhc" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2hhc, resolution 1.54&Aring;" />
'''Crystal structure of fucosyltransferase NodZ from Bradyrhizobium'''<br />


==Overview==
==Crystal structure of fucosyltransferase NodZ from Bradyrhizobium==
The alpha-1,6-fucosyltransferase NodZ from Bradyrhizobium sp. WM9 (Lupinus), composed of 325 amino acids with a molecular weight of 37 kDa, has been cloned, expressed and purified. Protein crystals suitable for X-ray diffraction were obtained under optimized crystallization conditions using ammonium dihydrogen phosphate as a precipitant. The crystals are hexagonal and belong to space group P6(1)22 or P6(5)22, with unit-cell parameters a = 125.5, c = 95.6 A, and contain 56.8% solvent and a single protein molecule in the asymmetric unit. Native data were collected to 2.85 A using synchrotron radiation and cryogenic conditions. The native crystals were soaked in a mother-liquor solution containing 2.5 mM [Ta(6)Br(12)](2+) cluster for derivatization and SAD data were collected to 3.4 A at the tantalum L(III) absorption peak.
<StructureSection load='2hhc' size='340' side='right'caption='[[2hhc]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hhc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_sp._WM9 Bradyrhizobium sp. WM9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HHC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hhc OCA], [https://pdbe.org/2hhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hhc RCSB], [https://www.ebi.ac.uk/pdbsum/2hhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hhc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9AQ17_BRASW Q9AQ17_BRASW]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hhc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hhc ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2HHC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_sp. Bradyrhizobium sp.] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glycoprotein_6-alpha-L-fucosyltransferase Glycoprotein 6-alpha-L-fucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.68 2.4.1.68] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHC OCA].
*[[NodS|NodS]]
 
__TOC__
==Reference==
</StructureSection>
Cloning, purification, crystallization and preliminary crystallographic studies of Bradyrhizobium fucosyltransferase NodZ., Brzezinski K, Rogozinski B, Stepkowski T, Bujacz G, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):344-6. Epub 2004, Jan 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14747720 14747720]
[[Category: Bradyrhizobium sp. WM9]]
[[Category: Bradyrhizobium sp.]]
[[Category: Large Structures]]
[[Category: Glycoprotein 6-alpha-L-fucosyltransferase]]
[[Category: Brzezinski K]]
[[Category: Single protein]]
[[Category: Bujacz G]]
[[Category: Brzezinski, K.]]
[[Category: Jaskolski M]]
[[Category: Bujacz, G.]]
[[Category: Panjikar S]]
[[Category: Jaskolski, M.]]
[[Category: Stepkowski T]]
[[Category: Panjikar, S.]]
[[Category: Stepkowski, T.]]
[[Category: PO4]]
[[Category: TRS]]
[[Category: fucosyltransferase]]
[[Category: glycosyltransferase]]
[[Category: nodulation]]
[[Category: nodz]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:41:53 2008''

Latest revision as of 16:52, 13 March 2024

Crystal structure of fucosyltransferase NodZ from BradyrhizobiumCrystal structure of fucosyltransferase NodZ from Bradyrhizobium

Structural highlights

2hhc is a 1 chain structure with sequence from Bradyrhizobium sp. WM9. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.54Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9AQ17_BRASW

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2hhc, resolution 1.54Å

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