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New page: '''Unreleased structure''' The entry 4ko1 is ON HOLD Authors: Volbeda, A., Cavazza, C., Fontecilla-Camps, J.C. Description: High X-ray dose structure of H2-activated anaerobically puri... |
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==High X-ray dose structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air== | |||
<StructureSection load='4ko1' size='340' side='right'caption='[[4ko1]], [[Resolution|resolution]] 1.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ko1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfomicrobium_baculatum Desulfomicrobium baculatum] and [https://en.wikipedia.org/wiki/Desulfomicrobium_baculatum_DSM_4028 Desulfomicrobium baculatum DSM 4028]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KO1 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FCO:CARBONMONOXIDE-(DICYANO)+IRON'>FCO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ko1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ko1 OCA], [https://pdbe.org/4ko1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ko1 RCSB], [https://www.ebi.ac.uk/pdbsum/4ko1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ko1 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PHSS_DESBA PHSS_DESBA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
This study shows how the NiFeSe site of an anaerobically purified O2-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape. | |||
Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase.,Volbeda A, Amara P, Iannello M, De Lacey AL, Cavazza C, Fontecilla-Camps JC Chem Commun (Camb). 2013 Jul 11;49(63):7061-3. doi: 10.1039/c3cc43619e. PMID:23811828<ref>PMID:23811828</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4ko1" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Desulfomicrobium baculatum]] | |||
[[Category: Desulfomicrobium baculatum DSM 4028]] | |||
[[Category: Large Structures]] | |||
[[Category: Cavazza C]] | |||
[[Category: Fontecilla-Camps JC]] | |||
[[Category: Volbeda A]] | |||
Latest revision as of 19:02, 20 September 2023
High X-ray dose structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under airHigh X-ray dose structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air
Structural highlights
FunctionPublication Abstract from PubMedThis study shows how the NiFeSe site of an anaerobically purified O2-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape. Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase.,Volbeda A, Amara P, Iannello M, De Lacey AL, Cavazza C, Fontecilla-Camps JC Chem Commun (Camb). 2013 Jul 11;49(63):7061-3. doi: 10.1039/c3cc43619e. PMID:23811828[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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