3of1: Difference between revisions

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-{{STRUCTURE_3of1|  PDB=3of1  |  SCENE=  }}
-===Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA===
-{{ABSTRACT_PUBMED_21070946}}
-==Function==
+==Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA==
-[[http://www.uniprot.org/uniprot/KAPR_YEAST KAPR_YEAST]] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.<ref>PMID:3037314</ref> <ref>PMID:2823100</ref> <ref>PMID:6243658</ref> <ref>PMID:12704202</ref>
+<StructureSection load='3of1' size='340' side='right'caption='[[3of1]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3of1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OF1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3of1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3of1 OCA], [https://pdbe.org/3of1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3of1 RCSB], [https://www.ebi.ac.uk/pdbsum/3of1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3of1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/KAPR_YEAST KAPR_YEAST] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.<ref>PMID:3037314</ref> <ref>PMID:2823100</ref> <ref>PMID:6243658</ref> <ref>PMID:12704202</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The major cAMP receptors in eukaryotes are the regulatory (R) subunits of PKA, an allosteric enzyme conserved in fungi through mammals. While mammals have four R-subunit genes, Saccharomyces cerevisiae has only one, Bcy1. To achieve a molecular understanding of PKA activation in yeast and to explore the evolution of cyclic-nucleotide binding (CNB) domains, we solved the structure of cAMP-bound Bcy1(168-416). Surprisingly, the relative orientation of the two CNB domains in Bcy1 is very different from mammalian R-subunits. This quaternary structure is defined primarily by a fungi-specific sequence in the hinge between the alphaB/alphaC helices of the CNB-A domain. The unique interface between the two CNB domains in Bcy1 defines the allosteric mechanism for cooperative activation of PKA by cAMP. Some interface motifs are isoform-specific while others, although conserved, play surprisingly different roles in each R-subunit. Phylogenetic analysis shows that structural differences in Bcy1 are shared by fungi of the subphylum Saccharomycotina.
-==About this Structure==
+Structure of yeast regulatory subunit: a glimpse into the evolution of PKA signaling.,Rinaldi J, Wu J, Yang J, Ralston CY, Sankaran B, Moreno S, Taylor SS Structure. 2010 Nov 10;18(11):1471-82. PMID:21070946<ref>PMID:21070946</ref>
-[[3of1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OF1 OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3of1" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[CAMP-dependent protein kinase|CAMP-dependent protein kinase]]
+*[[CAMP-dependent protein kinase 3D structures|CAMP-dependent protein kinase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021070946</ref><references group="xtra"/><references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Moreno, S.]]
+[[Category: Moreno S]]
-[[Category: Ralston, C Y.]]
+[[Category: Ralston CY]]
-[[Category: Rinaldi, J.]]
+[[Category: Rinaldi J]]
-[[Category: Sankaran, B.]]
+[[Category: Sankaran B]]
-[[Category: Taylor, S S.]]
+[[Category: Taylor SS]]
-[[Category: Wu, J.]]
+[[Category: Wu J]]
-[[Category: Yang, J.]]
+[[Category: Yang J]]
-[[Category: Camp-dependent protein kinase]]
-[[Category: Cyclic nucleotide binding domain]]
-[[Category: Evolution]]
-[[Category: Pka signaling]]
-[[Category: Regulatory subunit]]
-[[Category: Transferase]]