|
|
| (15 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:2ezv.gif|left|200px]]<br /><applet load="2ezv" size="350" color="white" frame="true" align="right" spinBox="true"
| |
| caption="2ezv, resolution 2.400Å" />
| |
| '''Crystal structure of tetrameric restriction endonuclease SfiI bound to cognate DNA.'''<br />
| |
|
| |
|
| ==Overview== | | ==Crystal structure of tetrameric restriction endonuclease SfiI bound to cognate DNA.== |
| Many reactions in cells proceed via the sequestration of two DNA molecules in a synaptic complex. SfiI is a member of a growing family of restriction enzymes that can bind and cleave two DNA sites simultaneously. We present here the structures of tetrameric SfiI in complex with cognate DNA. The structures reveal two different binding states of SfiI: one with both DNA-binding sites fully occupied and the other with fully and partially occupied sites. These two states provide details on how SfiI recognizes and cleaves its target DNA sites, and gives insight into sequential binding events. The SfiI recognition sequence (GGCCNNNN[downward arrow]NGGCC) is a subset of the recognition sequence of BglI (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer and BglI is a dimer, and there is little sequence similarity between the two enzymes, their modes of DNA recognition are unusually similar.
| | <StructureSection load='2ezv' size='340' side='right'caption='[[2ezv]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[2ezv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fimbriatus Streptomyces fimbriatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EZV FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ezv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ezv OCA], [https://pdbe.org/2ezv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ezv RCSB], [https://www.ebi.ac.uk/pdbsum/2ezv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ezv ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/T2S1_STRFI T2S1_STRFI] Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9. |
|
| |
|
| ==About this Structure== | | ==See Also== |
| 2EZV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZV OCA].
| | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| | | __TOC__ |
| ==Reference==
| | </StructureSection> |
| A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA., Vanamee ES, Viadiu H, Kucera R, Dorner L, Picone S, Schildkraut I, Aggarwal AK, EMBO J. 2005 Dec 7;24(23):4198-208. Epub 2005 Nov 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16308566 16308566]
| | [[Category: Large Structures]] |
| [[Category: Single protein]] | | [[Category: Streptomyces fimbriatus]] |
| [[Category: Type II site-specific deoxyribonuclease]] | | [[Category: Aggarwal AK]] |
| [[Category: Aggarwal, A K.]] | | [[Category: Vanamee ES]] |
| [[Category: Vanamee, E S.]] | | [[Category: Viadiu H]] |
| [[Category: Viadiu, H]] | |
| [[Category: CA]]
| |
| [[Category: protein/dna complex]]
| |
| [[Category: type iif restriction endonuclease]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:16:13 2008''
| |