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[[Image:2ex5.gif|left|200px]]<br /><applet load="2ex5" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ex5, resolution 2.200&Aring;" />
'''Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA'''<br />


==Overview==
==Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA==
Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.
<StructureSection load='2ex5' size='340' side='right'caption='[[2ex5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ex5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_moewusii Chlamydomonas moewusii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EX5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ex5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex5 OCA], [https://pdbe.org/2ex5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ex5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ex5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DNE1_CHLMO DNE1_CHLMO] Endonuclease involved in intron homing. Recognizes a degenerate sequence of 17-19 bp to produce a staggered cut 5 bp downstream from the CeLSU.5 intron insertion site.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/2ex5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ex5 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2EX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_eugametos Chlamydomonas eugametos] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold., Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL, Structure. 2006 May;14(5):869-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16698548 16698548]
[[Category: Chlamydomonas moewusii]]
[[Category: Chlamydomonas eugametos]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Synthetic construct]]
[[Category: Spiegel, P C.]]
[[Category: Spiegel PC]]
[[Category: Stoddard, B L.]]
[[Category: Stoddard BL]]
[[Category: CA]]
[[Category: homing endonuclease]]
[[Category: homodimer]]
[[Category: laglidadg]]
[[Category: protein/dna complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:15:29 2008''

Latest revision as of 12:20, 14 February 2024

Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNAGroup I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA

Structural highlights

2ex5 is a 4 chain structure with sequence from Chlamydomonas moewusii and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DNE1_CHLMO Endonuclease involved in intron homing. Recognizes a degenerate sequence of 17-19 bp to produce a staggered cut 5 bp downstream from the CeLSU.5 intron insertion site.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2ex5, resolution 2.20Å

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