NAD synthase: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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+<StructureSection load='' size='350' side='right' caption='NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry [[1xng]])' scene='54/541072/Cv/1'>
-[[Image:1ee1.png|left|200px|thumb|Crystal structure of NAD+ synthase complex with Mg+2, ATP and deamino-NAD  [[1ee1]]]]
-{{STRUCTURE_1ee1|  PDB=1ee1  | SIZE=350| SCENE= |right|CAPTION= NAD+ synthase complex with Mg+2, ATP and deamino-NAD [1ee1]] }}
+== Function ==
+'''NAD+ synthase''' or '''NH3-dependent NAD synthetase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH<sub>3</sub> to AMP, NAD+ and diphosphate (PP).  NADS is part of the NAD synthesis pathway<ref>PMID:12898714</ref> .  See [[NAD]].
+== Structural highlights ==
+The <scene name='54/541072/Cv/8'>ATP binding site</scene> contains <scene name='54/541072/Cv/9'>Mg+2 ion</scene>. The <scene name='54/541072/Cv/10'>NAD binding site is located in the interface of the 2 subunits</scene><ref>PMID:15645437</ref>. Water molecules are shown as red spheres.
+</StructureSection>
+== 3D Structures of NAD+ synthase ==
+Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
+*'''NAD synthase'''
+**[[3p52]] – NADS – ''Campylobacter jejuni'' <br />
+**[[3q4g]] – NADS (mutant) – ''Vibrio cholerae'' <br />
+**[[1wxf]] – EcNADS  – ''Escherichia coli'' <br />
+**[[1xnh]] – HpNADS  - ''Helicobacter pylori'' <br />
+**[[2pzb]] – BaNADS  - ''Bacillus anthracis'' <br />
+**[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br />
+**[[6kv3]] – NADS – ''Staphylococcus aureus''<br />
+**[[5wp0]] – NADS – ''Allivibrio fischeri''<br />
+**[[5huh]], [[5huj]] – NADS – ''Streptococcus pyogenes''<br />
+**[[3n05]] – NADS – ''Streptomyces avermitillis''<br />
+**[[4xfd]] – PaNADS – ''Pseudomonas aeruginosa''<br />
+**[[4q16]] – NADS – ''Deinococcus radiodurans''<br />
+**[[2e18]] – NADS – ''Pyrococcus horikoshii''<br />
+*NADS binary complex
+**[[1ifx]] – BsNADS + deamino-NAD  – ''Bacillus subtilis'' <br />
+**[[1wxh]] – EcNADS + NAD  <br />
+**[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br />
+**[[6c8q]] – NADS + NAD – ''Enterococcus faecalis''<br />
+**[[5f23]] – PaNADS + NAD <br />
+*NADS ternary complex
+**[[1ih8]] – BsNADS + Mg + ATP derivative  <br />
+**[[1wxe]] – EcNADS + Mg + AMP   <br />
+**[[1wxg]] – EcNADS + Mg + deamino-NAD  <br />
+**[[2pz8]] – BaNADS + Mg + ATP derivative  <br />
+**[[3fiu]] – NADS + Mg + AMP – ''Francisella tularensis''<br />
+*NADS quaternary complex
+**[[1ee1]] – BsNADS + Mg + ATP + deamino-NAD  <br />
+**[[1fyd]] – BsNADS + Mg + AMP + PP <br />
+**[[1kqp]] – BsNADS + Mg + PP + NAD  derivative<br />
+**[[1wxi]] – EcNADS + Mg + AMP + PP <br />
+**[[1xng]] – HpNADS + Mg + ATP + deamino-NAD  <br />
+**[[2pza]] – BaNADS + Mg + AMP + PP <br />
+*NADS quinary complex
+**[[1nsy]] – BsNADS + Mg + ATP + ADP + PP <br />
+**[[2nsy]] – BsNADS + Mg + AMP + NAD + PP <br />
+*NADS glutamine-dependent
+**[[6ofb]] – hNADS + AMP + NAD derivative<br />
+**[[3sdb]] – MtNADS (mutant) – ''Mycobacterium tuberculosis'' <br />
+**[[3seq]], [[3sez]] – MtNADS (mutant) + ATP derivative + pyridinium derivative <br />
+**[[3szg]] – MtNADS (mutant) + AMP + pyridinium derivative <br />
+**[[3syt]] – MtNADS + AMP + NAD + glutamate <br />
+**[[3dla]] – MtNADS + norleucine + deamido-NAD <br />
+**[[6ofc]] – MtNADS (mutant) + AMP + sulfonamide derivative + glutamate <br />
+**[[4f4h]] – NADS – ''Burkholderia thailandensis''<br />
+**[[3ilv]] – NADS – ''Cytophaga hutchinsonii''<br />
+}}
+== References ==
+<references/>
+[[Category:Topic Page]]
-'''NAD+ synthase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH3 to AMP, NAD+ and diphophate (PP).  NADS is part of the NAD synthesis pathway.
-== 3D Structures of NAD+ synthase ==
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-[[3p52]] – NADS – ''Campylobacter jejuni'' <br />
-[[3q4g]] – NADS (mutant) – ''Vibrio cholerae'' <br />
-[[1wxf]] – EcNADS  – ''Escherichia coli'' <br />
-[[1xnh]] – HpNADS  - ''Helicobacter pylori'' <br />
-[[2pzb]] – BaNADS  - ''Bacillus anthracis'' <br />
-[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br />
-===NADS binary complex===
-[[1ifx]] – BsNADS + deamino-NAD  – ''Bacillus subtilis'' <br />
-[[1wxh]] – EcNADS + NAD  <br />
-[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br />
-===NADS ternary complex===
-[[1ih8]] – BsNADS + Mg + ATP derivative  <br />
-[[1wxe]] – EcNADS + Mg + AMP   <br />
-[[1wxg]] – EcNADS + Mg + deamino-NAD  <br />
-[[2pz8]] – BaNADS + Mg + ATP derivative  <br />
-===NADS quaternary complex===
-[[1ee1]] – BsNADS + Mg + ATP + deamino-NAD  <br />
-[[1fyd]] – BsNADS + Mg + AMP + PP <br />
-[[1kqp]] – BsNADS + Mg + PP + NAD  derivative<br />
-[[1wxi]] – EcNADS + Mg + AMP + PP <br />
-[[1xng]] – HpNADS + Mg + ATP + deamino-NAD  <br />
-[[2pza]] – BaNADS + Mg + AMP + PP <br />
-[[1nsy]] – BsNADS + Mg + ATP + ADP + PP <br />
-[[2nsy]] – BsNADS + Mg + AMP + NAD + PP <br />