2axx: Difference between revisions

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New page: left|200px<br /> <applet load="2axx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2axx" /> '''THE SOLUTION STRUCTURE OF OXIDIZED RAT MICR...
 
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[[Image:2axx.gif|left|200px]]<br />
<applet load="2axx" size="450" color="white" frame="true" align="right" spinBox="true"
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'''THE SOLUTION STRUCTURE OF OXIDIZED RAT MICROSOMAL CYTOCHROME B5, NMR, 21 STRUCTURES'''<br />


==Overview==
==THE SOLUTION STRUCTURE OF OXIDIZED RAT MICROSOMAL CYTOCHROME B5, NMR, 21 STRUCTURES==
The solution structure of oxidized rat microsomal cytochrome b5 has been, obtained from 1H NMR spectra measured at 800 MHz. The available assignment, has been extended to 78% of the total protons and 95% of the residues., From 1372 meaningful NOEs, a family of 40 structures has been obtained, through the program DYANA; 235 pseudocontact shifts have been then added, as further constraints, obtaining an essentially similar family of, structures. This latter family has been further refined through restrained, energy minimization. The final RMSD values with respect to the average, structure are 0.58 +/- 0.10 A and 1.05 +/- 0.11 A for backbone and heavy, atoms, respectively. The high quality of the structure allows meaningful, comparisons with the solution structure of the reduced protein, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9425037 (full description)]]
<StructureSection load='2axx' size='340' side='right'caption='[[2axx]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2axx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AXX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2axx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2axx OCA], [https://pdbe.org/2axx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2axx RCSB], [https://www.ebi.ac.uk/pdbsum/2axx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2axx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CYB5_RAT CYB5_RAT] Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-6 double bond introduction during the C-6 desaturation.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ax/2axx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2axx ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2AXX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]] with HEM as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AXX OCA]].
*[[Cytochrome b5 3D structures|Cytochrome b5 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The solution structure of oxidized rat microsomal cytochrome b5., Arnesano F, Banci L, Bertini I, Felli IC, Biochemistry. 1998 Jan 6;37(1):173-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9425037 9425037]
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Arnesano F]]
[[Category: Arnesano, F.]]
[[Category: Banci L]]
[[Category: Banci, L.]]
[[Category: Bertini I]]
[[Category: Bertini, I.]]
[[Category: Felli IC]]
[[Category: Felli, I.C.]]
[[Category: HEM]]
[[Category: cytochrome b5]]
[[Category: electron transfer]]
[[Category: electron transport]]
[[Category: paramagnetic nmr]]
[[Category: protein recognition]]
[[Category: solution structure]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 16:27:19 2007''

Latest revision as of 09:37, 1 May 2024

THE SOLUTION STRUCTURE OF OXIDIZED RAT MICROSOMAL CYTOCHROME B5, NMR, 21 STRUCTURESTHE SOLUTION STRUCTURE OF OXIDIZED RAT MICROSOMAL CYTOCHROME B5, NMR, 21 STRUCTURES

Structural highlights

2axx is a 1 chain structure with sequence from Rattus norvegicus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CYB5_RAT Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-6 double bond introduction during the C-6 desaturation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

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