3ki7: Difference between revisions

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{{STRUCTURE_3ki7|  PDB=3ki7  |  SCENE=  }}
===Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-I===


==Function==
==Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-I==
[[http://www.uniprot.org/uniprot/Q5EK40_VIBCH Q5EK40_VIBCH]] An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.<ref>PMID:18276581</ref> <ref>PMID:19793133</ref>
<StructureSection load='3ki7' size='340' side='right'caption='[[3ki7]], [[Resolution|resolution]] 1.32&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ki7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KI7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.32&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G9I:2-{2-[4-(PYRROLIDIN-1-YLMETHYL)PHENYL]ETHYL}-5,6-DIHYDROIMIDAZO[4,5,1-JK][1,4]BENZODIAZEPIN-7(4H)-ONE'>G9I</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ki7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ki7 OCA], [https://pdbe.org/3ki7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ki7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ki7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ki7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CHXA_VIBCL CHXA_VIBCL] An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.<ref>PMID:18276581</ref> <ref>PMID:19793133</ref>  


==About this Structure==
==See Also==
[[3ki7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KI7 OCA].
*[[Exotoxin 3D structures|Exotoxin 3D structures]]
 
== References ==
==Reference==
<references/>
<references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
[[Category: Edwards, P R.]]
[[Category: Edwards PR]]
[[Category: Jorgensen, R.]]
[[Category: Jorgensen R]]
[[Category: Merrill, A R.]]
[[Category: Merrill AR]]
[[Category: Adp-ribosyl transferase]]
[[Category: Alpha-beta complex]]
[[Category: Diphthamide]]
[[Category: Toxin]]
[[Category: Transferase]]

Latest revision as of 11:16, 6 September 2023

Catalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-ICatalytic fragment of Cholix toxin from Vibrio Cholerae in complex with inhibitor GP-I

Structural highlights

3ki7 is a 1 chain structure with sequence from Vibrio cholerae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.32Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CHXA_VIBCL An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.[1] [2]

See Also

References

  1. ↑ Jorgensen R, Purdy AE, Fieldhouse RJ, Kimber MS, Bartlett DH, Rod Merrill A. Cholix toxin, a novel ADP-ribosylating factor from vibrio cholerae. J Biol Chem. 2008 Feb 25;. PMID:18276581 doi:M710008200
  2. ↑ Turgeon Z, White D, Jorgensen R, Visschedyk D, Fieldhouse RJ, Mangroo D, Merrill AR. Yeast as a tool for characterizing mono-ADP-ribosyltransferase toxins. FEMS Microbiol Lett. 2009 Nov;300(1):97-106. Epub 2009 Aug 31. PMID:19793133 doi:10.1111/j.1574-6968.2009.01777.x

3ki7, resolution 1.32Å

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