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| [[Image:2dtr.jpg|left|200px]]<br /><applet load="2dtr" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2dtr, resolution 1.9Å" />
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| '''STRUCTURE OF DIPHTHERIA TOXIN REPRESSOR'''<br />
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| ==Overview== | | ==STRUCTURE OF DIPHTHERIA TOXIN REPRESSOR== |
| The crystal structure of diphtheria toxin repressor (DtxR) in complex with the corepressor Co2+ has been determined at 2.0 A resolution and in complex with Mn2+ at 2.2 A resolution. The structure of the flexible third domain could be determined at this high resolution. It appears to contain five antiparallel strands exhibiting a fold very similar to the SH3 domain. A superposition of 46 equivalent C alpha atoms of DtxR and alpha-spectrin SH3 resulted in an rms deviation of 3.0 A. The sequence identity is only 7%. This third domain of DtxR appears to have no interactions with the DNA binding domain nor with the metal binding domain of the repressor. Yet, flexibility in the region between the second and the third domain allows in principle significant conformational changes such as might occur upon DNA binding. The two metal binding sites in the second domain have been unraveled in considerable detail. Metal binding site 1 was well occupied in both the cobalt and manganese structures and showed a surprising sulfate ion as ligand. The sulfate was proven beyond doubt by the high peak at its position in a selenate versus sulfate difference Fourier. The presence of the intriguing sulfate ion at such a crucial position near the metal corepressor suggests the possibility that under physiological conditions phosphate may act as a "co-corepressor" for this class of metal-regulated DNA binding proteins in Corynebacteria, Mycobacteria, and related organisms. The second metal binding site is significantly different in these two DtxR structures. In the 2.0 A cobalt structure, the site is not occupied by a metal ion. In the 2.2 A manganese structure the site is well occupied, at approximately the same position as observed previously in cadmium DtxR. The ligands are Glu105, His106, the carbonyl oxygen of Cys102, and a water molecule. The reasons for differential occupancy of this site in different structures are intriguing and require further investigations.
| | <StructureSection load='2dtr' size='340' side='right'caption='[[2dtr]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[2dtr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1dtr 1dtr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DTR FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dtr OCA], [https://pdbe.org/2dtr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dtr RCSB], [https://www.ebi.ac.uk/pdbsum/2dtr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dtr ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/DTXR_CORDI DTXR_CORDI] Iron-binding repressor of the dipheteria toxin gene expression. May serve as a global regulator of gene expression. Represses ripA under iron excess. |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/2dtr_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dtr ConSurf]. |
| | <div style="clear:both"></div> |
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| ==About this Structure== | | ==See Also== |
| 2DTR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=CO:'>CO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1DTR. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DTR OCA].
| | *[[Diphtheria toxin repressor|Diphtheria toxin repressor]] |
| | | __TOC__ |
| ==Reference==
| | </StructureSection> |
| High-resolution structure of the diphtheria toxin repressor complexed with cobalt and manganese reveals an SH3-like third domain and suggests a possible role of phosphate as co-corepressor., Qiu X, Pohl E, Holmes RK, Hol WG, Biochemistry. 1996 Sep 24;35(38):12292-302. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8823163 8823163]
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| [[Category: Corynebacterium diphtheriae]] | | [[Category: Corynebacterium diphtheriae]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Hol, W G.]] | | [[Category: Hol WG]] |
| [[Category: Pohl, E.]] | | [[Category: Pohl E]] |
| [[Category: Qiu, X.]] | | [[Category: Qiu X]] |
| [[Category: CO]]
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| [[Category: SO4]]
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| [[Category: dna-binding]]
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| [[Category: iron]]
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| [[Category: repressor]]
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| [[Category: transcription regulation]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:02:31 2008''
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