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[[Image:2cwb.gif|left|200px]]<br /><applet load="2cwb" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2cwb" />
'''Solution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with Ubiquitin'''<br />


==Overview==
==Solution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with Ubiquitin==
Ubiquitin is an important cellular signal that targets proteins for degradation or regulates their functions. The previously identified BMSC-UbP protein derived from bone marrow stromal cells contains a ubiquitin-associated (UBA) domain at the C terminus that has been implicated in linking cellular processes and the ubiquitin system. Here, we report the solution NMR structure of the UBA domain of human BMSC-UbP protein and its complex with ubiquitin. The structure determination was facilitated by using a solubility-enhancement tag (SET) GB1, immunoglobulin G binding domain 1 of Streptococcal protein G. The results show that BMSC-UbP UBA domain is primarily comprised of three alpha-helices with a hydrophobic patch defined by residues within the C terminus of helix-1, loop-1, and helix-3. The M-G-I motif is similar to the M/L-G-F/Y motifs conserved in most UBA domains. Chemical shift perturbation study revealed that the UBA domain binds with the conserved five-stranded beta-sheet of ubiquitin via hydrophobic interactions with the dissociation constant (KD) of approximately 17 microM. The structural model of BMSC-UbP UBA domain complexed with ubiquitin was constructed by chemical shift mapping combined with the program HADDOCK, which is in agreement with the result from mutagenesis studies. In the complex structure, three residues (Met76, Ile78, and Leu99) of BMSC-UbP UBA form a trident anchoring the domain to the hydrophobic concave surface of ubiquitin defined by residues Leu8, Ile44, His68, and Val70. This complex structure may provide clues for BMSC-UbP functions and structural insights into the UBA domains of other ubiquitin-associated proteins that share high sequence homology with BMSC-UbP UBA domain.
<StructureSection load='2cwb' size='340' side='right'caption='[[2cwb]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2cwb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CWB FirstGlance]. <br>
2CWB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._and_homo_sapiens Streptococcus sp. and homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWB OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwb OCA], [https://pdbe.org/2cwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cwb RCSB], [https://www.ebi.ac.uk/pdbsum/2cwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cwb ProSAT]</span></td></tr>
==Reference==
</table>
Solution structure of the ubiquitin-associated domain of human BMSC-UbP and its complex with ubiquitin., Chang YG, Song AX, Gao YG, Shi YH, Lin XJ, Cao XT, Lin DH, Hu HY, Protein Sci. 2006 Jun;15(6):1248-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16731964 16731964]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/UBL7_HUMAN UBL7_HUMAN]
[[Category: Streptococcus sp. and homo sapiens]]
== Evolutionary Conservation ==
[[Category: Cao, X T.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Chang, Y G.]]
Check<jmol>
[[Category: Gao, Y G.]]
  <jmolCheckbox>
[[Category: Hu, H Y.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/2cwb_consurf.spt"</scriptWhenChecked>
[[Category: Lin, D H.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Lin, X J.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Shi, Y H.]]
  </jmolCheckbox>
[[Category: Song, A X.]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cwb ConSurf].
[[Category: helical bundle]]
<div style="clear:both"></div>
 
__TOC__
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:53:03 2008''
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Streptococcus sp. 'group G']]
[[Category: Cao XT]]
[[Category: Chang YG]]
[[Category: Gao YG]]
[[Category: Hu HY]]
[[Category: Lin DH]]
[[Category: Lin XJ]]
[[Category: Shi YH]]
[[Category: Song AX]]

Latest revision as of 09:39, 1 May 2024

Solution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with UbiquitinSolution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with Ubiquitin

Structural highlights

2cwb is a 1 chain structure with sequence from Homo sapiens and Streptococcus sp. 'group G'. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UBL7_HUMAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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