DNA Polymerase I: Difference between revisions
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<StructureSection load='1KLN_pymol.pdb' size='400' side='right' scene='Sandbox_dvoet/DNA_polymerase/Klenow-dna/4' caption='' > | <StructureSection load='1KLN_pymol.pdb' size='400' side='right' scene='Sandbox_dvoet/DNA_polymerase/Klenow-dna/4' caption='' > | ||
==Overview== | ==Overview== | ||
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==Structure of the whole ''Thermus aquaticus'' DNA polymerase I== | ==Structure of the whole ''Thermus aquaticus'' DNA polymerase I== | ||
As mentioned above, Pol I's primary and essential function is to excise the RNA primers from newly synthesized Okazaki fragments with its 5' → 3' exonuclease function and replace them with DNA using its polymerase function. This yields a double-stranded DNA (dsDNA) with a single strand nick between successive Okazaki fragments, a nick that is eventually sealed through the action of DNA ligase. | As mentioned above, Pol I's primary and essential function is to excise the RNA primers from newly synthesized Okazaki fragments with its 5' → 3' exonuclease function and replace them with DNA using its polymerase function. This yields a double-stranded DNA (dsDNA) with a single strand nick between successive Okazaki fragments, a nick that is eventually sealed through the action of DNA ligase. | ||