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==Native fungus laccase from Trametes hirsuta== | |||
<StructureSection load='3fpx' size='340' side='right'caption='[[3fpx]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3fpx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trametes_hirsuta Trametes hirsuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPX FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpx OCA], [https://pdbe.org/3fpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpx RCSB], [https://www.ebi.ac.uk/pdbsum/3fpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpx ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B2L9C1_TRAHI B2L9C1_TRAHI] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpx_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpx ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Laccase from the fungus Coriolus hirsutus has been purified. Crystals of the enzyme suitable for X-ray structure analysis have been obtained under optimized crystallization conditions using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.65, b = 74.01, c = 124.83 A, and contain 40% solvent and a single molecule of laccase in the asymmetric unit. X-ray data were collected to 1.85 A at the copper edge and the four copper sites have been located from the anomalous signal. The obtained SAD phases with subsequent density modification produced a promising initial electron-density map. | |||
Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus.,Pegasova TV, Zwart P, Koroleva OV, Stepanova EV, Rebrikov DV, Lamzin VS Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1459-61. Epub 2003, Jul 23. PMID:012876350<ref>PMID:012876350</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3fpx" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[Laccase 3D structures|Laccase 3D structures]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Trametes hirsuta]] | [[Category: Trametes hirsuta]] | ||
[[Category: Cherkashin | [[Category: Cherkashin EA]] | ||
[[Category: Fedorova | [[Category: Fedorova TV]] | ||
[[Category: Koroleva | [[Category: Koroleva OV]] | ||
[[Category: Kurzeev | [[Category: Kurzeev SA]] | ||
[[Category: Lamzin | [[Category: Lamzin VS]] | ||
[[Category: Polyakov | [[Category: Polyakov KM]] | ||
[[Category: Stepanova | [[Category: Stepanova EV]] | ||
[[Category: Strokopytov | [[Category: Strokopytov BV]] | ||
Latest revision as of 12:09, 30 October 2024
Native fungus laccase from Trametes hirsutaNative fungus laccase from Trametes hirsuta
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedLaccase from the fungus Coriolus hirsutus has been purified. Crystals of the enzyme suitable for X-ray structure analysis have been obtained under optimized crystallization conditions using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.65, b = 74.01, c = 124.83 A, and contain 40% solvent and a single molecule of laccase in the asymmetric unit. X-ray data were collected to 1.85 A at the copper edge and the four copper sites have been located from the anomalous signal. The obtained SAD phases with subsequent density modification produced a promising initial electron-density map. Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus.,Pegasova TV, Zwart P, Koroleva OV, Stepanova EV, Rebrikov DV, Lamzin VS Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1459-61. Epub 2003, Jul 23. PMID:012876350[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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