4ilh: Difference between revisions

Latest revision as of 18:25, 20 September 2023

Crystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseHCrystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseH

Structural highlights

4ilh is a 2 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.85Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRP8_YEAST Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.^[1] ^[2] ^[3] ^[4]

References

↑ Jackson SP, Lossky M, Beggs JD. Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing. Mol Cell Biol. 1988 Mar;8(3):1067-75. PMID:2835658
↑ Abovich N, Rosbash M. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell. 1997 May 2;89(3):403-12. PMID:9150140
↑ McPheeters DS, Muhlenkamp P. Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast. Mol Cell Biol. 2003 Jun;23(12):4174-86. PMID:12773561
↑ Yang K, Zhang L, Xu T, Heroux A, Zhao R. Crystal structure of the beta-finger domain of Prp8 reveals analogy to ribosomal proteins. Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13817-22. Epub 2008 Sep 8. PMID:18779563

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OCA

[1] Jackson SP, Lossky M, Beggs JD. Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing. Mol Cell Biol. 1988 Mar;8(3):1067-75. PMID:2835658

[2] Abovich N, Rosbash M. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell. 1997 May 2;89(3):403-12. PMID:9150140

[3] McPheeters DS, Muhlenkamp P. Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast. Mol Cell Biol. 2003 Jun;23(12):4174-86. PMID:12773561

[4] Yang K, Zhang L, Xu T, Heroux A, Zhao R. Crystal structure of the beta-finger domain of Prp8 reveals analogy to ribosomal proteins. Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13817-22. Epub 2008 Sep 8. PMID:18779563

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[2]

[3]

[4]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4ilh is ON HOLD  until Paper Publication
+==Crystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseH==
+<StructureSection load='4ilh' size='340' side='right'caption='[[4ilh]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4ilh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ILH FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ilh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilh OCA], [https://pdbe.org/4ilh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ilh RCSB], [https://www.ebi.ac.uk/pdbsum/4ilh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ilh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>
-Authors: Weber, G., Heroven, A.C., Santos, K.F., Wahl, M.C.
+==See Also==
+*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
-Description: Crystal structure of an Aar2 C-terminal deletion mutant in conplex with Prp8 RNaseH
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Saccharomyces cerevisiae S288C]]
+[[Category: Heroven AC]]
+[[Category: Santos KF]]
+[[Category: Wahl MC]]
+[[Category: Weber G]]

4ilh: Difference between revisions

Latest revision as of 18:25, 20 September 2023

Crystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseHCrystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseH

Structural highlights

Function

See Also

References

Contents

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4ilh: Difference between revisions

Latest revision as of 18:25, 20 September 2023

Crystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseHCrystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseH

Structural highlights

Function

See Also

References

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