3eqa: Difference between revisions

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-[[Image:3eqa.png|left|200px]]
-{{STRUCTURE_3eqa|  PDB=3eqa  |  SCENE=  }}
+==Catalytic domain of glucoamylase from aspergillus niger complexed with tris and glycerol==
+<StructureSection load='3eqa' size='340' side='right'caption='[[3eqa]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3eqa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EQA FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eqa OCA], [https://pdbe.org/3eqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eqa RCSB], [https://www.ebi.ac.uk/pdbsum/3eqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eqa ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AMYG_ASPNG AMYG_ASPNG]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eq/3eqa_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eqa ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Glucoamylase from Aspergillus niger is an industrially important biocatalyst that is utilized in the mass production of glucose from raw starch or soluble oligosaccharides. The G1 isoform consists of a catalytic domain and a starch-binding domain connected by a heavily glycosylated linker region. The amino-terminal catalytic domain of the G1 isoform generated by subtilisin cleavage has been crystallized at pH 8.5, which is a significantly higher pH condition than used for previously characterized glucoamylase crystals. The refined structure at 1.9 A resolution reveals the active site of the enzyme in complex with both Tris and glycerol molecules. The ligands display both unique and analogous interactions with the substrate-binding site when compared with previous structures of homologous enzymes bound to inhibitors.
-===Catalytic domain of glucoamylase from aspergillus niger complexed with tris and glycerol===
+Structure of the catalytic domain of glucoamylase from Aspergillus niger.,Lee J, Paetzel M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Feb 1;67(Pt, 2):188-92. Epub 2011 Jan 21. PMID:21301084<ref>PMID:21301084</ref>
-{{ABSTRACT_PUBMED_21301084}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3eqa" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[3eqa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQA OCA].
+*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021301084</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Aspergillus niger]]
-[[Category: Glucan 1,4-alpha-glucosidase]]
+[[Category: Large Structures]]
-[[Category: Lee, J.]]
+[[Category: Lee J]]
-[[Category: Paetzel, M.]]
+[[Category: Paetzel M]]
-[[Category: Glycoprotein]]
-[[Category: Glycosidase]]
-[[Category: Hydrolase]]
-[[Category: Polysaccharide degradation]]