2i68: Difference between revisions

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[[Image:2i68.png|left|200px]]


{{STRUCTURE_2i68|  PDB=2i68  |  SCENE=  }}
==Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE==
 
<StructureSection load='2i68' size='340' side='right'caption='[[2i68]], [[Resolution|resolution]] 7.50&Aring;' scene=''>
===Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I68 FirstGlance]. <br>
{{ABSTRACT_PUBMED_17005200}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 7.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [https://pdbe.org/2i68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [https://www.ebi.ac.uk/pdbsum/2i68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i68 ProSAT]</span></td></tr>
==About this Structure==
</table>
[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA].  
== Function ==
 
[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
==Reference==
== Evolutionary Conservation ==
<ref group="xtra">PMID:017005200</ref><ref group="xtra">PMID:014633977</ref><references group="xtra"/>
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i68_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i68 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Ben-Tal, N.]]
[[Category: Large Structures]]
[[Category: Enosh, A.]]
[[Category: Ben-Tal N]]
[[Category: Fleishman, S J.]]
[[Category: Enosh A]]
[[Category: Halperin, D.]]
[[Category: Fleishman SJ]]
[[Category: Harrington, S E.]]
[[Category: Halperin D]]
[[Category: Tate, C G.]]
[[Category: Harrington SE]]
[[Category: Dual topology]]
[[Category: Tate CG]]
[[Category: Homodimer]]
[[Category: Small-multidrug resistance]]
[[Category: Transmembrane protein]]
[[Category: Transport protein]]
[[Category: Transporter]]

Latest revision as of 16:52, 13 March 2024

Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrECryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE

Structural highlights

2i68 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron crystallography, Resolution 7.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EMRE_ECOLI Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.[1] [2] [3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Yerushalmi H, Lebendiker M, Schuldiner S. EmrE, an Escherichia coli 12-kDa multidrug transporter, exchanges toxic cations and H+ and is soluble in organic solvents. J Biol Chem. 1995 Mar 24;270(12):6856-63. PMID:7896833
  2. ↑ Yerushalmi H, Schuldiner S. An essential glutamyl residue in EmrE, a multidrug antiporter from Escherichia coli. J Biol Chem. 2000 Feb 25;275(8):5264-9. PMID:10681497
  3. ↑ Rotem D, Schuldiner S. EmrE, a multidrug transporter from Escherichia coli, transports monovalent and divalent substrates with the same stoichiometry. J Biol Chem. 2004 Nov 19;279(47):48787-93. Epub 2004 Sep 15. PMID:15371426 doi:10.1074/jbc.M408187200

2i68, resolution 7.50Å

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