3zi5: Difference between revisions
New page: '''Unreleased structure''' The entry 3zi5 is ON HOLD Authors: Golovenko, D., Manakova, E., Zakrys, L., Zaremba, M., Sasnauskas, G., Grazulis, S., Siksnys, V. Description: Crystal STRUC... |
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The | ==Crystal STRUCTURE OF RESTRICTION ENDONUCLEASE BFII C-TERMINAL RECOGNITION DOMAIN IN COMPLEX WITH COGNATE DNA== | ||
<StructureSection load='3zi5' size='340' side='right'caption='[[3zi5]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3zi5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Cytobacillus_firmus Cytobacillus firmus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZI5 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zi5 OCA], [https://pdbe.org/3zi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zi5 RCSB], [https://www.ebi.ac.uk/pdbsum/3zi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zi5 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9F4C9_CYTFI Q9F4C9_CYTFI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The B3 DNA-binding domains (DBDs) of plant transcription factors (TF) and DBDs of EcoRII and BfiI restriction endonucleases (EcoRII-N and BfiI-C) share a common structural fold, classified as the DNA-binding pseudobarrel. The B3 DBDs in the plant TFs recognize a diverse set of target sequences. The only available co-crystal structure of the B3-like DBD is that of EcoRII-N (recognition sequence 5'-CCTGG-3'). In order to understand the structural and molecular mechanisms of specificity of B3 DBDs, we have solved the crystal structure of BfiI-C (recognition sequence 5'-ACTGGG-3') complexed with 12-bp cognate oligoduplex. Structural comparison of BfiI-C-DNA and EcoRII-N-DNA complexes reveals a conserved DNA-binding mode and a conserved pattern of interactions with the phosphodiester backbone. The determinants of the target specificity are located in the loops that emanate from the conserved structural core. The BfiI-C-DNA structure presented here expands a range of templates for modeling of the DNA-bound complexes of the B3 family of plant TFs. | |||
Structural insight into the specificity of the B3 DNA-binding domains provided by the co-crystal structure of the C-terminal fragment of BfiI restriction enzyme.,Golovenko D, Manakova E, Zakrys L, Zaremba M, Sasnauskas G, Grazulis S, Siksnys V Nucleic Acids Res. 2014 Jan 13. PMID:24423868<ref>PMID:24423868</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3zi5" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Endonuclease 3D structures|Endonuclease 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cytobacillus firmus]] | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Golovenko D]] | |||
[[Category: Grazulis S]] | |||
[[Category: Manakova E]] | |||
[[Category: Sasnauskas G]] | |||
[[Category: Siksnys V]] | |||
[[Category: Zakrys L]] | |||
[[Category: Zaremba M]] | |||